A pre-lysis buffer washing procedure was introduced to DNA extraction from a forest soil with high organic matter and iron oxide contents. Sodium phosphate of 0.1 M (pH 7.5) was used as a buffer to wash soil samples when subsequent lysis buffer was phosphate, and 20 mM EDTA (pH 7.5) ...
Note 5:Pre-Sort Buffer isnota post-sort collection buffer. Please collect cells in buffer/media that is appropriate for downstream applications. Note 6:Pre-Sort Buffer is compatible with commonly used viability dyes such as 7-AAD. 显示较多信息 ...
Additionally, for MN, an incubation step with PBS (Phosphate Buffered Saline) buffer at high temperature prior to lysis and a purification step post extraction were evaluated using a fixed-effect model of two factors with interaction. DNA concentrations and purity ratios were...