Here, we investigate the role of Syntaxin7 (Stx7) in the release of lytic granules from cytotoxic T lymphocytes (CTLs). We show that Stx7 is expressed in CTLs and is preferentially localized to the region of lytic granule release, the immunological synapse (IS). Interference of Stx7 ...
Here, we investigate the role of Syntaxin7 (Stx7) in the release of lytic granules from cytotoxic T lymphocytes (CTLs). We show that Stx7 is expressed in CTLs and is preferentially localized to the region of lytic granule release, the immunological synapse (IS). Interference of Stx7 ...
2e). Interestingly, γδD4 T cells also had more chondroitin sulfate, which is associated with serglycin, a core protein within the dense core of cytotoxic granules, (Fig. 2d, e and Supplementary Fig. 2f) but the same level of CD63, a late endosomal/lysosomal marker, (Supplementary Fig...
EETosis releases intact granules and DNA nets predominantly free of granule proteins Neutrophils, diverse granule-derived proteins, many with antimicrobial activities, have been recognized to be bound as free proteins to extracellular DNA nets.16,33 Likewise, for the elicited release of mitochondrial ...
(mean ± SD; 10 cells/sample). The results show that before biotin addition GZMB has a low co-localization with lytic granules (marked by LAMP-1), while after biotin addition the co-localization increases in a time-dependent fashion, indicating that after release from the ER GZMB is ...
Eosinophil extracellular DNA trap cell death mediates lytic release of free secretion-competent eosinophil granules in humans. Blood 121, 2074-2083, doi: ... S Ueki,RCN Melo,I Ghiran,... - 《Blood》 被引量: 63发表: 2013年 Eosinophil Extracellular DNA Trap Cell Death (ETosis) Mediates Lyti...
Using anti-granzyme B labelling of sectioned cells in combination with electron microscopy, we confirmed that granzyme B was mostly localised in dense-core granules. We compared Vγ9Vδ2 T cells that had been expanded for 4 days (γδD4, amid peaking granzyme B expression) or 14 days (γ...
Ligand-bound integrins, predominantly the αLβ2 isoform LFA-1, function as spatial cues to attract lytic granules containing perforin and granzyme and induce their fusion with the plasma membrane for content release. LFA-1 is subjected to pulling forces within secretory domains, and disruption of...
Cytotoxic T cells assemble a LFA-1/talin ring junction through which lytic granules are secreted (Somersalo et al., 2004, Stinchcombe et al., 2001). Lytic granules have been shown to release their contents by fusing with an actin-depleted secretory domain located toward the synapse center (Ri...
adaptor protein 1 (AP-1) sorting complex, also causes retention of perforin in the transport vesicles and inhibits cytotoxicity, indicating that the interaction between AP-1 sorting complex and LAMP1 on the surface of the transport vesicles is important for perforin trafficking to lytic granules. ...