3A and E). We further inoculated mCherry-labelled SiHa cells into the footpads of mice. When the tumour reached 50 mm3, CAF-CM or NF-CM was then randomly injected into the centre of the xenograft tumours twice a week. After 4 weeks, the CAF-CM-treated group showed a significantly ...
CD31+PDPN+ cells were considered to be LECs and were sorted using the FACS Aria Fusion (Beckton Dickinson) system. Sorted LECs were directly placed into each well in a 96-well plate containing a lysis buffer. The plates were snap-frozen with liquid nitrogen and stored at −80 °C. ...
administration route and physico-chemical responsiveness. The recent focus in PAI is on targeting the lymphatic system and nodes for cancer diagnosis, as they play a vital role in cancer progression and metastasis. This review aims to discuss the clinical advancements of PAI using nanoparticles as e...
The labelled LECs were solubilized in MTT solvent and the absorbance was measured at 570 nm with a reference filter of 620 nm. Cell migration assay. Cell migration was examined using QCM 24-Well Colorimetric Cell Migration Kit (8 mm pore size; Millipore). Briefly, conditioned media from LPS-...
it can be induced that the SLN may be the major gateway for metastasis to the distant sites. Nathanson et al. suggest that breast cancer cells may invade the lymphatic system at least most of the time rather than the blood vessels at the primary site. Once the breast cancer cells reach ...
9. It can be seen that equilibration of labelled albumin between the blood and the afferent lymph (i.e. the tissue fluid of the tumour) had taken place within 7 h. The corresponding result from the experi- ment on the normal sheep was 9 h. In both sheep the prefemoral lymph took ...
(Figs.5e,6g). However, since it is generally considered that naïve T cells migrate via blood to LN, while activated and memory T cell migrate via tissues and lymph to LN46, future investigations should determine how the LT system regulates the migration of activated and memory CD4 and ...
45 years ago, neutrophils were detected within the lymphatic system13. It was suggested that this response represented a way for neutrophils to recirculate back into the blood vasculature; and their role in the lymphatic system was thus largely neglected. Recently, however, accumulating evidence has...
liver diseases, such as viral hepatitis and liver cancer. In addition, optimization of the formulation of the enteral delivery system should probably allow reducing the therapeutic doses. On the other hand, the long-term effects and potential side effects of the delivery system remain to be ...