5.Mix 20µl of cell lysate with 100µl of Luciferase Assay Reagent and measure the light produced.Preparation of Plant Tissue Lysate 1.Quick-freeze the tissue in liquid nitrogen, grind the frozen tissue to a powder and resuspend in room temper-ature 1X lysis reagent with homogenization.2...
Dual-Luciferase Reporter Assay System 星级: 26 页 protocol_2_ 96 well Luciferase and Renilla assay_updated 2 星级: 2 页 Application of the dual-luciferase reporter assay to the analysis of promoter activity in Zebrafish embryos 星级: 8 页 steady-Glo luciferase system 星级: 24 页 [PPT...
Secreted luciferase assay protocol eliminates cell lysis 2–4 fold higher signal than firefly or Renilla luciferase Single transfection allows multiple time points from same cell Dual, live-cell assay includes SEAP as well as secreted luciferase: monitor two promoters, or use one reporter as a...
Nano-Glo® Dual-Luciferase® Reporter Assay System Injectors ProtocolRELATED PRODUCTSNano-Glo® Dual-Luciferase Reporter Assay System Need Assistance Contact us Download Video: Closed Format: MP4 Open Format: Video Description The Nano-Glo® Dual-Luciferase® Rep...
Including coenzyme A in the reaction enhances the sensitivity of the assay and provides a sustained light reaction. In this protocol, cells transfected with a luciferase reporter plasmid are lysed using a detergent-containing buffer. Cell debris is removed by ...
Luciferase Assay System 简明操作指导.pdf,Quick PROTOCOL Luciferase Assay System 简明操作指导 注意:这是节选操作步骤,详细英文说明书见 适用产品目录号:E1483,E1500,E1501,E1531, /protocols/ E4030,E4530,E4550 I. 需用户自备的材料 • 不透光多孔白板或发光检测
Luciferase Reporter Assay KitCatalog Number KA
The assay protocol was run and analyzed as described above. Cited references Williams, T.M., et al. (1989). Advantages of firefly luciferase as a reporter gene: application to the interleukin-2 gene promo...
Assay. Prior to beginning this protocol, verify that the LAR II and the Stop & Glo? Reagent have been warmed to room temperature. The assays for firefly luciferase activity and Renilla luciferase activity are performed sequentially using one reaction tube. The following protocol is designed for ...
Kit components: Qty 1 tube 1 tube 200 uL 10 mL 1 Description GoClone™ positive control GoClone™ negative control FuGENE HD tfx reagent LightSwitch Assay Reagent™ Optimization Protocol Storage -20°C for 6 months -20°C for 6 months 4°C for 6 months -20°C for 6 months Genome ...