Lipids are a major form of energy storage in animals. They are stored in the intracellular neutral lipid droplets of specialized tissues such as adipose tissue in mammals and the fat body in insects. Although initially found in fat-related tissues, lipid droplets are organelles present in many,...
Mesenteric lymph nodes were harvested, with care to remove any remaining fat, and stored in cold PBS with 10% FCS. Harvested lymph nodes were passed through a 70-μm cell strainer to obtain a single-cell suspension. Cell numbers and viability were assessed using Trypan blue in an EVE ...
while at the same time, the microbiota shifts from a few pioneering species in low abundance towards a complex microbial ecosystem with a large load1. In addition, during this time, the immune system is educated so it provides tolerance
Quantitative real‑time PCR (qRT‑PCR) About 0.02 g of tissue stored in Trizol was put into a new 1.5 mL centrifuge tube. 1 mL Trizol was added to the homogenizer (BioPrep-24, Allsheng China) to grind the homogenate thoroughly. After mixing, the homogen- ate was cracked...
Quantitative real‑time PCR (qRT‑PCR) About 0.02 g of tissue stored in Trizol was put into a new 1.5 mL centrifuge tube. 1 mL Trizol was added to the homogenizer (BioPrep-24, Allsheng China) to grind the homogenate thoroughly. After mixing, the homogen- ate was cracked...