The objective was to evaluate the ability of sorghum phenolic extract to inhibit inflammasomes in THP-1 human macrophages. THP-1 human macrophages was pre-treated with sorghum phenolics and the inflammasome was activated by lipopolysaccharide and adenosine triphosphate treatment. Treatment of macrophages ...
Cells containing predominantly either LMP subunits (such as THP-1 and human monocytes), or only X, Y, Z subunits (RAW 264.7 cells not primed) could only induce TNF-α, but not NO, while cells containing all five to six subunits (XYZ, LMP) of the proteasome could induce both mediators ...
(F and G) THP-1 cells were primed with IFN-γ and LPS, separately, followed by LPS and IFN-γ treatment, respectively, for 24h. Ccl2 mRNA and protein expression was quantified and statistically analyzed as described above. The data show that only the IFN-γ priming followed by LPS ...
(F and G) THP-1 cells were primed with IFN-γ and LPS, separately, followed by LPS and IFN-γ treatment, respectively, for 24h. Ccl2 mRNA and protein expression was quantified and statistically analyzed as described above. The data show that only the IFN-γ priming followed by LPS ...
Fig. 1: IFNγ priming is required for LPS-induced caspase-4 activation in human epithelial cells. a–c Intracellular bacterial fold-replication (a) and release of LDH (b, c) in naive or IFNγ-primed wild-type, CASP4–/– or GSDMD–/– HeLa cells, at 1 or 6-h post-infection (p....
cGAS-stimulation was naturally sustained by undigested self-nucleic acids in fibroblasts from DNase2-deficiency interferonopathy, whilst it was pharmacologically obtained by cGAMP-stimulation in THP1 cells and murine bone marrow-derived dendritic cells. We showed that cells with a primed cGAS-STING-...
35. As little as 1 ng/ml has been demonstrated to trigger necrotic and apoptotic monocyte death36and intrinsic and extrinsic apoptosis in the monocytic cell line THP-137. Indeed, Liu and colleagues demonstrated the involvement of the mitochondrial apoptotic pathway in LPS-related death, although...
Figure 1. LY6E downregulates CD14 protein expression and attenuates the LPS-induced inflammatory response (A) THP-1 cells were infected with lentivirus encoding control or LY6E-specific shRNA and subjected to immunoblotting analysis of CD14, LY6E and GADPH as the loading control. R.S.I., rela...
1C–E). NLRP3, ASC, pro-IL-1β, and caspase-1 (p45) expression in whole cell lysates were not affected by CDN (Fig. 1C). Similarly, PMA-primed THP-1 cells were pretreated with CDN and then stimulated with nigericin. Results also showed that caspase-1 cleavage and mature IL-1β ...
The objective was to evaluate the ability of sorghum phenolic extract to inhibit inflammasomes in THP-1 human macrophages. THP-1 human macrophages was pre-treated with sorghum phenolics and the inflammasome was activated by lipopolysaccharide and adenosine triphosphate treatment. Treatment of macrophages ...