But the effects and mechanisms of DHEA on pyroptosis remain unclear. Here, we found that DHEA inhibited the NLRP3 inflammasome components expression by blocking inflammatory signals in lipopolysaccharide (LPS)-primed macrophages, and prevented the bacterial toxin nigericin (Nig)-induced NLRP3 ...
We next primed THP-1 cells with LPS (1 μg/mL) for 4 h followed by Nigericin (5 μg/mL) stimulation to induce inflammasome and pyroptosis. LPS/Ng treatment triggered a higher level of mitochondrial ROS (Fig. 1A, B, shTFG vs. shTFG LPS/Ng) and promoted pyroptotic cell death (Fig...
目的研究脂多糖(LPS)联合尼日利亚菌素(Nigericin,Nig)不同剂量下对肝细胞的损伤作用及Z-DNA结合蛋白1(Z-DNA Binding Protein 1,ZBP1)在肝细胞焦亡(Pyroptosis)中发挥... 余丽娜 - 《华北理工大学》 被引量: 0发表: 2023年 Nimbolide通过调节HDAC3缓解自身免疫性肝炎小鼠肝内炎症的作用与机制研究 则LPS诱导的AML...
Results: Periodontal ligament fibroblasts and DPCs were more sensitive to cytoplasmic LPS‐induced noncanonical pyroptosis than to canonical pyroptosis induced by stimulation with LPS priming plus nigericin or by poly(dA:dT) transfection. In addition, treatment with DMF attenuated cytoplasmic LPS‐induced ...
In addition, LPS-induced cell damage, pyroptosis, and fibrosis were aggravated after Nigericin treatment, while PNS alleviated the aggravation caused by Nigericin.Conclusion: PNS inhibited pyroptosis by inhibiting the activation of NLRP3 inflammasome in LPS-induced HK-2 cells, which ultimately alleviated...
Cells were treated with or without LPS in the presence and absence of nigericin. All proteins were normalized to the level of β-actin. Data represent mean ± SEM, n = 3, * p < 0.05, ** p < 0.01. Physiological roles for GSDMD in both pyroptosis and IL-1β release during ...