通过作用于细胞膜,nigericin可引起细胞内离子平衡失调,导致线粒体功能障碍和氧化应激,从而诱导细胞死亡。 一般来说,nigericin可以通过两种方式诱导焦亡:一种是通过刺激细胞内线粒体通透性转变,导致线粒体释放细胞色素C等凋亡相关蛋白;另一种是通过激活caspase-1和下游的促凋亡通路。研究表明,nigericin诱导的焦亡过程中,...
, human leukemic monocytes (THP-1 cells, Tohoku Hospital Pediatrics-1 cells) were preincubated with different concentrations of grape/blueberry anthocyanins, homovanillyl alcohol, or 2,4,6-trihydroxybenzaldehyde (THBA) before the NLRP3 inflammasome was activated by lipopolysaccharide and/or nigericin....
Immunoblots of IL-1β, caspase-1 in the supernatant and lysate of (a) Pggt1bfl/fl Lyz2-Cre, Pggt1bfl/fl Lyz2-Cre Casp-1−/−, Pggt1b+/+ Casp1+/+ or Casp-1−/− BMDMs stimulated with LPS or LPS and Nigericin; (b) Pggt1b+/+ Pycard+/+, Pycard−/−, Pggt1bf...
The NLRP3 inflammasome activation requires two signals [6]: one being the transcriptional priming such as LPS and another one being ATP, nigericin, or monosodium urate (MSU) crystals. NLRP3 upon activation, processes the pro-casp1 into mature casp1, activating gasdermin D (GSDMD) that form ...
4A, Munronoid I could suppress the production of LPS/nigericin-induced IL-1β, IL-6 and IL-18 in a dose-dependent manner. We further investigated whether Munronoid I could reduce the pro-inflammatory cytokines production in a time-dependent manner. As shown in Fig. 4B, Munronoid I ...
Here, we reported that TFG promotes the stabilization of Unc-51 like autophagy activating kinase (ULK1) and participates in LPS plus nigericin (Ng) induced pyroptotic cell death. Our results showed that TFG-deficient THP-1 macrophages exhibit higher mitochondrial ROS production. LPS/Ng stimulation...
We aimed to to determine the role of neutrophil-NLRP3 and neutrophil aging in LPS-induced mortality in P4 MKO pups. Serum IL-1尾 was measured 4h post-LPS injection. BM neutrophils were treated with LPS and nigericin for NLRP3 protein expression in lysate and IL-1b levels in the ...
Results: Periodontal ligament fibroblasts and DPCs were more sensitive to cytoplasmic LPS‐induced noncanonical pyroptosis than to canonical pyroptosis induced by stimulation with LPS priming plus nigericin or by poly(dA:dT) transfection. In addition, treatment with DMF attenuated cytoplasmic LPS‐induced ...
E Control or TFG-knockdown THP-1 cells were treated with LPS (1 μg/mL) for 4 h followed by Nigericin (5 μg/mL) stimulation for 60 min. Whole-cell lysates and supernatants were subjected to IB. Experiments were independently repeated three times. inflammation and pyroptotic cell death ...
To examine whether obtusifolin affected NLRP3 inflammasome activation, BV2 cells were pretreated with LPS and then stimulated with ATP or nigericin. The secretion of IL-1尾 was measured by ELISA, whereas the expression of NLRP3 inflammasome-related proteins was detected using Western blotting. The...