在 PSG-1 存在下,肠样 Caco-2/RAW264.7 共培养模型中,炎症刺激后测量 TNF-α、Il-6、IL-1β 的水平。与图1 A 中所示的 TNF-α、IL-6 和 IL-1β 分泌的结果相似,肠样 Caco-2/RAW264.7 共培养炎症模型中,向顶室...
研究表明,适量的L-色氨酸具有保护和修复被LPS破坏的Caco-2细胞单层屏障功能的作用。饮食L-色氨酸显著减轻损害紧密连接并且使蛋白表达下调,可能通过激活ERK1/2-MAP通路和阻塞NFκB-MLCK信号通路的激活。因此,L-色氨酸可以作为食物、肠道...
实验先测定LPS诱导的RAW264.7巨噬细胞和肠样Caco-2/RAW264.7共培养模型中细胞因子的产生,以检测PSG-1的抗炎作用。首先确定在LPS诱导的RAW264.7巨噬细胞和肠样Caco-2/RAW264.7共培养模型中,PSG-1是否可以抑制LPS诱导的TNF-α、IL-6、IL-1β的分泌。
探讨了辣木多肽(MOPP)对脂多糖(LPS,2μg/mL)诱发Caco-2细胞高通透性的保护作用.MTT法测定细胞生存率,细胞乳酸脱氢酶(LDH)水平依说明用试剂盒测定.酶联法(ELISA)检测白介素-1β (IL-1β)、IL-8和肿瘤坏死因子(TNF-α)的分泌水平.跨上皮细胞电阻(TEER)值和异硫氰酸荧光素-右旋糖酐(FD40)透过度评估细胞...
This study utilized both a TLR4 reporter cell line and a Caco-2 IEC model to examine the effects of HM on lipopolysaccharide (LPS)-induced TLR4 activation and cytokine responses, respectively. Additionally, we performed fast protein liquid chromatography and mass spectrometry to identify a HM ...
目的:探讨蛇床子素(osthole)对内毒素(lipopolysaccharide,LPS)诱导的肠上皮细胞Caco2中炎症因子表达的影响及机制.方法:培养Caco2细胞,用LPS诱导炎症反应.在LPS刺激前给予细胞不同浓度的蛇床子素处理,通过real-time PCR检测白介素(interleukin,IL)-1β,IL-6和肿瘤坏死因子(tumor necrosis factor,TNF)-α的表达情况....
请问有做过LPS刺激caco-2细胞炎症的吗，我用100μg/mL的LPS刺激但是没有明显的炎症效果？LPS浓度达到...
They reported that Apixaban increased the expression of tumor suppression gene p16 in all cell lines, but reduced proliferation in only 3 cancer cell lines (OVCAR3, CaCO-2, and LNCaP) [42]. These results suggest that the same compound might exhibit different effects in different cell lines. ...
(CON) and LPS-challenged Caco-2, T84, and HT29 cells. Promonocytic THP-1 cells were used as positive controls. (B) Representative immunostaining of CD14 and TLR4 proteins in control (AandC) or LPS-exposed (B) cells. Confocal microscopic imaging inCindicates apical staining of CD14 or ...
表3 辣木多肽对LPS处理Caco-2细胞TEER与FD-40透过度的影响Table 3 Effects of MOPP on TEER and FD-40 levels in LPS treated intestinal Caco-2 cells 图3 辣木多肽对LPS处理Caco-2细胞中细胞间紧密连接相关因子的mRNA转录水平的影响Fig.3 Effects of MOPP on tight junction related factors mRNA expression ...