supporting the view that a DN result may represent aparticular time-point along the two curves of ATI titer rise and infliximab drug level decline.CONCLUSION:DN status may result from false negative detection of IFX or ATI by double antigen ELISA,suggesting a transitional state of low-level ...
We provided experimental data indicating that the extended V2 domain contributed to the higher level of resistance to neutralization by mothers' sera in this strain. Taken together the data suggest that some primary isolates with specific properties may be useful indicators for identifying protective ...
Host cells were distributed into the wells of a 96-well plate (1 ml per well, Axygen), and phage of a known titer were then added to an input concentration of 105 plaque-forming units per milliliter (PFU ml−1). The cultures were grown overnight (14–20 hours) with shaking...
Transwell invasion assay of Res186, Res259 and BT66 (BRAF fusion+) cells infected with miR-control or miR-125b, migrated cells were photographed (200x) by inverted light microscopy (A) or quantitated with Cell-Titer blue (B). All data were normalized to miR-control. (**p < 0.01...
5 minutes. Five minutes later, the number of infectious viral titer was determined using the same plaque assay as mentioned above. The number of viruses thus determined was regarded as the viral amount obtainable without the virus inactivating reaction, that is, the viral titer before inactivation...
Furthermore, the difference observed in measuring neutralizing antibodies between sVNT and RBD ELISA also suggested that the level of RBD-binding IgG antibodies used alone is not reliable for evaluating the level of neutralizing capacity in companion animals. Dileepan et al. showed that the neutral...
Obviously, in this case, the humoral immune response was affected to the extent of allowing the development of secondary bacterial infections at a very low feed level of OTA of 1 ppm (Figure 4). Another example of increased susceptibility to target microbial infection, e.g., Mycobacterium ...
In the present scenario, the high value of protein engineering has been well recognized in industrial-level biotransformation [100]. Protein engineering, with assistance from molecular approaches or directed evolution, rational design, or computational methods, enables the accelerated designing of ...
Samples were dissolved in 32 µL complete RPMI media, and viable AdHu5–PfRH5 was quantified using a hexon immunoassay (QuickTiter Immunoassay, Cambridge Bioscience, Cambridge, UK). 2.4. Immunization Female Balb/c mice, 4–6 weeks old (Harlan, Hillcrest, UK), were used in all exper- ime...
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