Using Arabidopsis thaliana root cells and specific antibodies raised against acyl-CoAs, we show that acyl-CoAs are mainly localized in endoplasmic reticulum membranes. Our results demonstrate the value of cryo-methods for the accurate localization of labile metabolites in plant cells....
SummaryThe effect of a series of long-chain acyl-CoAs and acylcarnitines has been tested by differential scanning calorimetry on the gel-fluid transition of saturated phosphatidylcholines and of dielai-doylphosphatidylethanolamine (DEPE), and on the lamellar-hexagonal transition of DEPE. Both series...
Antibodies directed against long chain acyl-CoAs (having 16 and 18 carbon atoms) have been prepared and are reported for the first time. A modified ELISA procedure adapted to these amphiphilic molecules has been developed: it is a rapid, simple and sensitive test permitting to detect as little...
D Neess,S Bek,H Engelsby,SF Gallego,NJ F?Rgeman 摘要: Long-chain fatty acyl-CoA esters are key intermediates in numerous lipid metabolic pathways, and recognized as important cellular signaling molecules. The intracellular flux and regulatory properties of acyl-CoA esters have been proposed to ...
Long-chain acyl-CoA esters are important intermediates in lipid metabolism and are synthesized from fatty acids by long-chain acyl-CoA synthetases (ACSL). The hematophagous insect Rhodnius prolixus , a vector of Chagas' disease, produces glycerolipids in the midgut after a blood meal, which ar...
Real-time PCR was also used to measure mRNA levels of other ACSL isoforms and FA transport protein (FATP) isoforms, at least some of which have acyl-CoA synthetase activity (Table 1). TABLE 1. Primers for real-time qPCR and semi-quantitative RT-PCR PrimerPrimer SequenceAmplicon Length ...
long-chain acyl-coa synthetase in fatty acid metabolism involved in liver and other diseases an update:长链酰基辅酶a合成酶在脂肪酸代谢中涉及肝脏和其他疾病的一个更新 文档格式: .pdf 文档大小: 945.55K 文档页数: 8页 顶/踩数: 0/0 收藏人数: ...
β1 subunit mutations that inhibit AMPK activation by the small-molecule activator A769662, which binds to the allosteric drug and metabolite site, also inhibit activation by LCFA-CoAs. Thus, LCFA-CoA metabolites act as direct endogenous AMPK β1–selective activators and promote LCFA oxidation....
1. 1. The activities of long-chain acyl-CoA synthetase (acid : CoA ligase (AMP-forming), EC 6.2.1.3) and the ‘outer’ carnitine long-chain acyltransferase (palmitoyl-CoA: l-carnitine O-palmitoyltransferase, EC 2.3.1.21) have been estimated in intact brown adipose tissue mitochondria. The ...
Previously we demonstrated that supplementation with the polyunsaturated fatty acids (PUFA) arachidonic acid (AA) or docosahexaenoic acid (DHA) increased neurite outgrowth of PC12 cells during differentiation, and that overexpression of rat acyl-CoA synthetase long-chain family member 6 (Acsl6, formerl...