Shrago E. Long-chain acyl-CoA as a multi-effector ligand in cellu- lar metabolism. J Nutr 130: 290S-293S, 2000.Shrago E 2000 Long-chain acyl-CoA as a multi-effector ligand in cellular metabolism. J Nutr 130:290S-293SShrago E. 2000. Long-chain acyl-CoA as a multi-effector ...
Antibodies directed against long chain acyl-CoAs (having 16 and 18 carbon atoms) have been prepared and are reported for the first time. A modified ELISA procedure adapted to these amphiphilic molecules has been developed: it is a rapid, simple and sensitive test permitting to detect as little...
Long-chain acyl-CoA esters are important intermediates in lipid metabolism and are synthesized from fatty acids by long-chain acyl-CoA synthetases (ACSL). The hematophagous insect Rhodnius prolixus , a vector of Chagas' disease, produces glycerolipids in the midgut after a blood meal, which a...
Long chain acyl-CoA synthetase (LACS), or long chain fatty acid-CoA ligase (FACL), converts free long chain fatty acids into fatty acyl-CoA esters, key intermediates in the synthesis of complex lipids. The FACL4 gene encodes a form of LACS and is express
Long-chain fatty acyl-CoA esters are key intermediates in numerous lipid metabolic pathways, and recognized as important cellular signaling molecules. The intracellular flux and regulatory properties of acyl-CoA esters have been proposed to be coordinated by acyl-CoA-binding domain containing proteins (...
Using PC12 cells undergoing neurite outgrowth, we studied the activation of various fatty acids, of different chain lengths and degrees of saturation, by long chain acyl-CoA synthetases (LCASs). Cells treated with nerve growth factor (NGF) were labeled with [3H]glycerol, [3H]oleic acid (OA)...
Real-time PCR was also used to measure mRNA levels of other ACSL isoforms and FA transport protein (FATP) isoforms, at least some of which have acyl-CoA synthetase activity (Table 1). TABLE 1. Primers for real-time qPCR and semi-quantitative RT-PCR PrimerPrimer SequenceAmplicon Length ...
long-chain acyl-CoA synthetases ACSs: acyl-CoA synthetases FAs: fatty acids TLCCM: total lipid content in culture medium qRT-PCR: quantitative reverse transcription-PCR GC/MASS: gas chromatography/mass spectrometry TAP: tris–acetate–phosphate DCW: dry cell weight C1-BODIPY-C12: 4,...
Previously we demonstrated that supplementation with the polyunsaturated fatty acids (PUFA) arachidonic acid (AA) or docosahexaenoic acid (DHA) increased neurite outgrowth of PC12 cells during differentiation, and that overexpression of rat acyl-CoA synthetase long-chain family member 6 (Acsl6, formerl...
1. 1. The activities of long-chain acyl-CoA synthetase (acid : CoA ligase (AMP-forming), EC 6.2.1.3) and the ‘outer’ carnitine long-chain acyltransferase (palmitoyl-CoA: l-carnitine O-palmitoyltransferase, EC 2.3.1.21) have been estimated in intact brown adipose tissue mitochondria. The ...