Light sheet fluorescence microscopy (LSFM), 3D image reconstruction and quantification processes A light sheet fluorescence microscope (Ultramicroscope II, Lavision Biotec, Bielefeld, Germany) and the Imspector™347 software were used. The microscope was equipped with an Olympus MVPLAPO 2x/0.5 objective...
However, when aligned over this ICK region, the N-terminal b-sheet of rDc1a is entirely separate to the region of structural overlap (Fig. 2c). Despite its structural uniqueness, the molecular surface of rDc1a contains a relatively uniform distribution of charged residues (Fig. 2d); more...
According to the recent BWS consensus, these features correspond to a score of 8 points that is consistent with the clinical diagnosis of classical BWS [4]. Her sister (III-2, Fig. 1a) was a 1-year-old child, born at 39 weeks of gestation with placental hyperplasia and polyhydramnios...
Basel, Switzerland) were seeded in 12-well plates (5×103cells per cm2) and differentiated into osteoblasts (using α-Mem pH7.5, 10% heat inactivated foetal calf serum (FCS), 100 nM Dexamethasone and 10 mM β-glycerophosphate). As mentioned in the datasheet provided by the company, c...