Monoclonal 类型 Antibody 克隆号 B.593.0 抗原 Synthetic peptide corresponding to the sequence of human LKB1 偶联物 Unconjugated 形式 Liquid 浓度 45 µg/mL 纯化类型 Affinity chromatography 保存液 0.01M HEPES, pH 7.5, with 0
Anti-Flag antibodies (M2 monoclonal and Flag polyclonal) were from Sigma. Anti-HA probe polyclonal antibody was from Santa Cruz. AICAR was obtained from Toronto Research Chemicals (Downsview, ON, Canada). Glucose-free media, D-PBS (containing 1 g/l D-glucose) and dialyzed serum was from ...
The immunocomplexes were probed by western blotting with monoclonal anti-LKB1 (Sigma-Aldrich), SIRT1 (Abcam), SIRT6 (Abcam), or acetyl-lysine antibody (Cell Signaling). Statistical analysis. Data are presented as means ± S.D. Two-way analysis of variance for comparisons was performed for ...
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Apoptotic cells were identified by double-stained with FITC-conjugated annexin-V monoclonal antibody and PI dye for 30 min by using the Annexin V-FITC Apoptosis Detection kit (BD Biosciences, 556547). After staining and washing, cells were analysed by LSR II cytometer. Apoptotic cells were ...
For analysis of Lkb1 levels, cell lysates were diluted to a final concentration of 0.5 mg/mL and loaded in a microplate (Protein Simple #043-165), along with a monoclonal mouse anti-Lkb1 antibody (1:50; Invitrogen 206R24). Western blotting was conducted according to the instructions for ...
Monoclonal U2OS cell lines stably expressing GFP-WIPI3S, GFPWIPI3 or GFP-WIPI4 have been generated upon transient transfections using Lipofectamine 2,000 (Invitrogen; 11668-019) and G418 selection (0.6 mg ml−1) (refs 31, 36). Autophagy assay Cells were incubated in full (fed, F)...
Rabbit monoclonal anti-GPx1 (clone EPR3311) antibody Abcam Cat#ab108429; RRID: AB_10865045 Rabbit monoclonal anti-AMPK-alpha phospho (Thr172) (clone 40H9) antibody Cell Signaling Technology Cat#2535; RRID: AB_331250 Rabbit monoclonal anti-AMPK alpha (clone D5A2) antibody Cell Signaling Technolo...
Monoclonal 类型 Antibody 克隆号 B.593.0 抗原 Synthetic peptide corresponding to the sequence of human LKB1 偶联物 Unconjugated 形式 Liquid 浓度 45 µg/mL 纯化类型 Affinity chromatography 保存液 0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol 内...
上海玉博生物科技有限公司 主要致力于“Mouse monoclonal [Ley 37D/G6] to LKB1 Abcam ab15095 ”的生产销售。多年的“Mouse monoclonal [Ley 37D/G6] to LKB1 Abcam ab15095 ”生产与销售的经验,与各行业新老用户建立了稳定的合作关系,我公司经营的产品名称深受广大用