A new assay using flow cytometry has been established to assess natural killer (NK) lytic activity with common bench top instrumentation. This assay uses a cyanine membrane dye to stain live K562 target cells and an iodide nuclear dye to evaluate dead cells, and provides a method of reliably ...
LIVE/DEAD™ 可固定远红外染色剂根据其在使用红色激光激发时提供明亮信号的荧光特性所选择。远红外荧光反应性染料的最大激发波长为 ∼633 nm,使其适用于与发射波长为 ∼655 nm 的红色或 HeNe 激光配合使用。因为可以使用流式细胞仪的单色染料和单通道来区分活细胞和死细胞、所以它是多色实验的理想选择。工作...
RayBiotech offers cell viability dyes in various fluorophores to detect live and dead cells during flow cytometry experiments. Browse the complete list here.
The Live or Dead™ Yeast Viability Assay Kit is a reliable and robust assay that allows researchers to quantitatively distinguish live and dead yeast within minutes using flow cytometry. This kit contains two ready-to-use nucleic acid stains: MycoLight™ Green JJ98, a replacement for SYTO™...
8. Analyze the stained cells by flow cytometry using violet (~405 nm) excitation and violet fluorescence emission (~450 nm) for the calcein violet (live cells) and blue-green fluorescence emission (~525 nm) for the aqua-fluorescent reactive dye (dead cells). Minimal compensation will be nece...
The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are based on the reaction of a fluorescent reactive dye with cellular amines. The reactive dye can permeate the compromised membranes of necrotic cells ...
The LIVE/DEAD™ Fixable dead cell stains were selected based on their fluorescent properties to provide a bright signal when excited with the optimal lasers. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, these dead cell stains are...
The LIVE/DEAD® Fixable Dead Cell Stain Kits use a novel method to evaluate the viability of mammalian cells by flow cytometry. These assays are based on the reaction of a fluorescent reactive dye with cellular amines. The reactive dye can permeate the compromised membranes of necrotic cells ...
The LIVE/DEAD™ Fixable dead cell stains were selected based on their fluorescent properties to provide a bright signal when excited with the optimal lasers. Since live and dead cells can be discriminated using a single dye and a single channel of a flow cytometer, these dead cell stains are...
By creating fluorescence spectral standard curves at 530 nm and 645 nm, the number of dead and live cells can be determined, and the fluorescence intensity of each dye is linearly related to the number of dead or live cells in the sample. Matters needing attention: 1. please centrifuge the...