RayBiotech offers cell viability dyes in various fluorophores to detect live and dead cells during flow cytometry experiments. Browse the complete list here.
Step 2: Use afixation and/or permeabilization buffer kitif staining with intracellular markers or to persevere stained cells.Do not use a fixation agent before the LIVE/DEAD reagent. Step 3: Stain cells with flow cytometry antibod...
Step 2: Use afixation and/or permeabilization buffer kitif staining with intracellular markers or to persevere stained cells.Do not use a fixation agent before the LIVE/DEAD reagent. Step 3: Stain cells with flow cytometry antibodies....
With these results, it became apparent that the combination with fluorogenic dyes and flow cytometry can provide a reliable test method to discriminate dead and/or injured cells from the reproductive live yeast cells, and is applicable for further studies. However, it should be noted that minimum...
SYTOX™ Dead Cell Stains are cell impermeant nucleic acid stains. By flow cytometry, dead cells can be easily distinguished from live cells by the increased fluorescent signal due to dsDNA binding of SYTOX™ dyes (Fig 1). Because they are non-fluorescent in aqueous media, SYTOX™ Dead Ce...
SYTOX™ Dead Cell Stain Sampler Kit contains five nucleic acid stains—SYTOX™ Blue, SYTOX™ Green, SYTOX™ Orange, SYTOX™ AADvanced™, SYTOX™ Red—that can be used to determine the viability of cells in flow cytometry assays. View a selection guide for all nonfixable viability ...
来源会议 23rd Annual Meeting of the Clinical-Cytometry-Society 2008/11/01 研究点推荐 TRYPAN BLUE FLOW CYTOMETRY LIVE/DEAD STAIN 0关于我们 百度学术集成海量学术资源,融合人工智能、深度学习、大数据分析等技术,为科研工作者提供全面快捷的学术服务。在这里我们保持学习的态度,不忘初心,砥砺前行。了解更多>> ...
流式细胞仪的cell sorting是很多细胞和分子生物实验的基础,比如单细胞测序、细胞周期等。 Flow cytometry and cell sorting are two distinct yet complementary techniques. Both rely on antibodies to detect specific cells within a heterogeneous population, but while flow cytometry measures the proportion of each...
Cells were washed with PBS for viability staining using LIVE/DEAD Fixable Violet Dead Cell Stain (Molecular Probes/Life Technologies, Carlsbad, CA, USA). FC experiments were done based on the manufacturer’s procedures for the FIX & PERM cell permeabilization kit (Life Technologies, Carlsbad, CA...
Its use in combination with flow cytometry brought up new questions about how to interpret LIVE/DEAD staining results. Intermediate states, normally difficult to detect with epifluorescence microscopy, are a common phenomenon when the assay is used in flow cytometry and still lack rationale. It is...