Nanoliposomes: preparation and analysis. Nanoliposomes: Preparation and analysis. In Liposomes: Methods and Protocols, Volume 1: Pharmaceutical Nanocarriers; Weissig, V., Ed.; Humana Press: New ... MR Mozafari - 《Methods in Molecular Biology》 被引量: 161发表: 2010年 Current trends in liposo...
Here, we describe the simultaneous formation and encapsulation of liposomes and various cell-mimetic lumen chemistries, respectively, using a 3D-printable microcapillary-based microfluidics device based off of the droplet-shooting and size-filtration (DSSF) liposome preparation method....
Here we report methods of preparation for liposome formulations containing lipophilic drugs. In contrast to the encapsulation of water soluble compounds into the entrapped aqueous volume of a...doi:10.1007/978-1-4939-6591-5_6Reto A. Schwendener...
protocol can be used for single- or double-stranded DNA orRNA, although most procedures have focused on single-stranded oligonucleotides. Not only can the naturally occurring DNA and RNA nucleotides be used in the SELEX protocol but modified nucleotides may be substituted. For modified nucleotides ...
These methods of liposome preparation are based on established protocols, but were modified here to exclude the use of detergent. In all liposome preparations we used L-α-phosphatidylcholine from soybean (type II-S, Sigma, abbreviated in the text as “asolectin”), which was washed as ...
specific preparation methods should be selected according to the liposome type to be obtained. Both bilayer constituents and charge of the vesicle govern the behavior of release of active molecules[86]. Once these systems are embedded in hydrogels, an additional release mechanism may be created. Wei...
Preparation of GGA GGA polymers were synthesized by following the protocol in a previous study27. Briefly, 5 g gelatin (Cat#V900863, Sigma, US) was dissolved in 150 mL DI water using a water bath at 40 °C. Simultaneously, 3.4 g gallic acid (GA, Cat#G823163, Macklin, Chin...
© 2017 Springer Science+Business Media New York About this protocol Cite this protocol Zhang, H. (2017). Thin-Film Hydration Followed by Extrusion Method for Liposome Preparation. In: D'Souza, G. (eds) Liposomes. Methods in Molecular Biology, vol 1522. Humana Press, New York, NY. https...
Protocol pp 187–207 Cite this protocol Affinity Biosensors Matthew A. Roberts& Richard A. Durst Part of the book series:Methods in Biotechnology((MIBT,volume 7)) 689Accesses Abstract During the last 10 years, there have been a number of applications of liposome reagents in various immunoasay...
(Polyethylene glycol 12-Glycerol Dimyristate). PEG stabilizes the liposomes by creating a steric barrier at the outer surface of the liposomes, the PEG chain has a molecular weight between about 300 Daltons and 5000 Daltons. Liposome preparation entails merely mixing the lipid with an aqueous ...