Stealth/siRNA Transfection Protocol Lipofectamine 2000 Reverse Transfection of Stealth RNAi or siRNA using Lipofectamine 2000 in a 96 well plate format siRNA Plasmid Co-Transfection Protocol with Lipofectamine 2000 Lipofectamine 2000 Diced siRNA Pools for Highly Effective RNAi Knockdown ...
siRNA transfection protocol 可在低siRNA浓度下获得优良的转染效果 在实现高效的基因抑制方面,Lipofectamine RNAiMAX试剂的表现可以轻松击败其他siRNA转染试剂。通过低至1 nM的siRNA,即可实现较高的靶标基因抑制水平(图1)。Lipofectamine RNAiMAX实验方案中建议采用10 nM的siRNA的起始量来获得最佳的基因敲低水平. 图1. ...
11668500 Lipofectamine™ 2000 Transfection Reagent, 15 mL Each 申請報價 - 實驗方案―Stealth RNAi或siRNA轉染 運用這個簡短的實驗流程,將Stealth RNAi或siRNA轉染到24孔盤規格的哺乳動物細胞中。所有的數量和體積都是以每單一孔為基礎單位。將本流程作為一個起點;依照《Stealth...
英文名称: Lipofectamine® RNAiMAX Transfection Reagent protocol 总访问: 416 国产/进口: 进口 半年访问: 16 产地/品牌: Invitrogen™ 产品类别: 细胞生物学试剂 规格: 1.5M; 最后更新: 2024-10-9 货号: Invitrogen™ CAS 号: 参考报价: 6700 立即询价 电话咨询 ...
siRNA transfection siRNA 3 pmol 7.5 pmol 15 pmol 30 pmol 75 pmol 166 pmol 434 pmol 592 pmol Lipofectamine 3000 reagent** 0.3 µL 0.75 µL 1.5 µL 3 µL 7.5 µL 17 µL 43 µL 59 µL T-175 92.11 35 mL 2 x 1.75 mL 46–96 µg 92–180 µL 46 µL 1,382...
Lipofectamine™ RNAiMAX 转染试剂可对多种细胞类型提供极高转染效率,以用于 siRNA 介导的基因敲低实验。Lipofectamine™ RNAiMAX 是专有的 RNAi特异性阳离子脂肪制剂,专为将了解更多信息 Have Questions? 更改视图 货号数量 137780750.75 mL 137781501.5 mL
Follow these general guidelines when using Lipofectamine™ 2000 to reverse transfect siRNA or Stealth™ RNAi duplexes into mammalian cells.• Use low-passage cells, and make sure that cells are healthy and greater than 90% viable before transfection. This protocol is for use with adherent ...
Find an optimized Lipofectamine® RNAiMAX transfection protocol for your cell line. 规格 Cell Type: Established Cell Lines, Hard-to-Transfect Cells, Primary Cells, Stem Cells Sample Type (Specific): Synthetic siRNA Transfection Technique: Lipid-Based Transfection Serum Compatible: Yes High Throughput...
200ng of two different reporter plasmids were co-transfected with 10pmol of siRNA following the standard Lipofectamine™ 2000 protocol, with 2ul of Lipofectamine™ 2000 per well. The total volume of the transfection mixes was 100ul, and it was added to the medium already in the wells. ...
产品名称:Lipofectamine® RNAiMAX Transfection Reagent 品牌名称:Life Technologies 所属分类:转染类 货号:13778150 规格:1.5 mL 目录价格:0 元产品描述 品牌介绍 同品牌产品 同分类产品 描述 Lipofectamine™ RNAiMAX转染试剂可在多种细胞类型中获得最高的转染效率,适用于siRNA介导的基因抑制实验。Lipofectamine™ ...