Lipofectamine 2000 is a cationic liposome based reagent that provides high transfection efficiency and high levels of transgene expression in a range of mammalian cell types in vitro using a simple protocol. Optimum transfection efficiency and subsequent cell viability depend on a number of experimental...
Lipofectamine™ 2000 CD(化学成分明确)转染试剂是一种无动物来源成分的专有配方,用于将核酸转染至真核细胞。使用 Lipofectamine™ 2000 CD转染试剂的优势如下:•在许多细胞类型和形式中具有极高的转染效率了解更多信息 Have Questions? 数量: 1 mL 货号12566014 ...
Invitrogen™ Lipofectamine™ 2000 Transfection Reagent is a proprietary formulation for the transfection of nucleic acids (DNA and RNA) into eukaryotic cells providing the following advantages: Highest transfection efficiency in many cell types and formats (e.g. 96-well). Refer to the Cell Line...
The inhibitors of endocytosis were used to treat HEp-2 cells before lipofectamine 2000/pGFP-N2 transfection. Transfection efficiency was evaluated with green fluorescence protein (GFP) expression assays. Cell viability and cytotoxicity were evaluated with MTT method. The results indicated that inhibitors ...
traditional Lipofectamine™ 2000 transfection protocols. In short, a different RNAi molecule is put in each well prior to transfection and combined with diluted Lipofectamine™ 2000 to form complexes in each well. Cells are added directly to the Lipofectamine™2000-RNA complexes and transfection ...
Optimizing Plasmid DNA Transfection To obtain the highest transfection efficiency and low cytotoxicity, optimize transfection conditions by varying cell density as well as DNA and Lipofectamine ™ 2000 concentrations. Make sure that cells are greater than 90% ...
However, the efficiency of complex formation may not be as high as with Opti-MEM. Cells in PBS can be transfected using Lipofectamine™ 2000. As long as the cells are healthy in the PBS, the transfection is likely to work. For a general plasmid DNA transfection protocol, please refer to...
2000,buttransfectionefficiencymay becompromised. 2.Testserum-freemediaformulationsforcompatibilitywithLipofectamine ™ 2000assomeserum-freeformulationscan inhibit cationic lipid-mediated transfection. CD 293, 293 SFM II, and VP-SFM are media formulations known to inhibit ...
Lipofectamine tm 2000说明书 Lipofectamine tm 2000 Cat.No.11668-027Size:0.75ml Cat.No.11668-019Sizes:1.5ml Lipofectamine2000isaproprietaryformulationsuitableforthetransfectionof nucleicacids(DNAandRNA)intoeukaryoticcellsprovideingthefollowing advantages: *Thehighesttransfectionefficiencyinmanycelltypesandformats(e...
When Lipofectamine® 2000 was utilized for the delivery of mRNAs into DRG neurons, a transfection efficiency as high as 25% was observed (based on EGFP mRNA transfection)(Williams et al., 2010). Besides that, further analysis validating the expression of several heterologous proteins namely, a...