Fig. 1. Upper panel: 2D display of mouse brain polar lipids as analyzed by LC–MS. Color-coded data are shown for clarity; original can be found in ref. [19]. The lipid classes from left to right are as follows: blue at ~7 min, PA; black, GalCer; gray, α-OH-GalCer; red, ...
brown – cerebellum. Sub-classes of the lipid predictors of long MLS linked to the enzymes in each functional group are listed next to the corresponding network module. The colors indicate the tissues where they are used as predictors of long MLS, according to the same color code as the GO...
The MS2 spectra shown in each panel are used as structural assessment by customized MFQL search files using the LipidXplorer search engine. Potential fragmentation patterns are shown above each panel. Annotated fragments are denoted in the chemical structure of the individual species. For lipid ...
The crystal structure of PLIN3 is shown on the farthest left panel (residues 191–437, PDB entry PDB:1SZI). PLIN2 homology model from (Najt et al., 2014) is shown in the second to the left panel, while the PLIN5 models are shown second from the right. Two structures, yellow and ...
CVD Risk Factors To assess changes in risk factors for CVD, blood pressure was taken along with a fasting lipid panel (total, LDL, and high-density lipoprotein cholesterol, and triglycerides), glucose, and insulin. Details on these procedures have been provided elsewhere.15 Dietary and Behavioral...
Then, 106 cells were resuspended in 25 µL of staining mix in 0.04% BSA-PBS containing APC-Cy7-labeled mouse anti-CD45 and the mouse cell surface protein antibody panel containing 151 oligo-conjugated antibodies (Supplementary Table 3; the CITE-seq antibody cocktail used in this study ...
[33]. Unfortunately, these studies did not link individual lipids to the generation of drug resistance. The initial MS Peak to Pathway-Metaboanalyst approach identified multiple pathways altered in the panel of prostate cancer cells analyzed. Hierarchical clustering found several nodes that correlated ...
c Left panel: Scatter plot of one-to-one comparison of the peptide numbers identified from TMT proteomics in (b and Supplementary Fig. 4b) for known MAM proteins between PNS (black circle) vs MAM (blue circle) fractions (**P < 0.01, two-tailed paired t test). Right: Bar graph ...
complete model of the seipin protein backbone (Fig.1cand Extended Data Fig.3f). This approach also allowed us to extend our model beyond what was resolved in the EM density map that contained almost all of the seipin sequence (conformation A, residues 11–283; conformation B, residues 8–...
The left graph (Panel B) shows the gene expression activities obtained in BMDCs with R8(SEQ ID NO: 4)-MEND and KALA(SEQ ID NO: 1)-MEND using pcDNA3.1-Luc(+) and pCpGfree-Luc(0) prepared in the section (a). The right graph (Panel C) shows the gene expression activities obtained...