Lipid Ontology enrichment analysis for lipidomics datasets
Lipidomics data statistical analysis; Supple- mentary Table S3. Lipid ontology enrichment; Supplemental Table S4. Couvillion et al. Microbiome (2023) 11:34 Page 18 of 20 Metabolomics data statistical analysis; Supplementary Table S5. Bray-Curtis PERMANOVA statistical analyses; Supplementary...
Metaboanalyst 6.0 was used to analyze the relative ion intensity for each lipid species due to sperm mobility phenotype through a t-test and due to timepoint through a one-way ANOVA, with lipid ontology enrichment analysis performed using LION. Metaboanalyst 6.0 was also used...
Gene ontology (GO) enrichment analysis Gene Ontology (GO) enrichment analysis was conducted for gene sets correlated with the mean lipid cluster profile drawn across 69 regions (based on Allen Brain data) with a coefficient greater than 0.5. For each category of GO terms (cellular compartment, ...
7D). To better understand these downregulated proteins, we performed Gene Ontology (GO) biological process (BP) enrichment analysis and found that many members of this group mapped to RNA splicing machinery (Supplementary Fig. 11b). Prior reports have found that IpaH9.8 binds to and inhibits U2...
To understand the downstream effects of lipid downregulation in SCA3, we conducted lipid ontology enrichment analysis through LION/web (Fig. 1C) (Molenaar et al., 2023; Molenaar et al., 2019; Ni et al., 2023). While lipid ontology resources are still in nascent stages, this provides a ba...
Unsupervised ontology-based analysis using LION (27) confirmed enrichment of metabotype 1 with positively-charged headgroup lipids and PCs specifically, as well as enrichment with plasmalogens, a minor HDL lipid class with antioxidant functions (30, 31) (Fig. 2E). Ontology-based analysis also ...
Additionally, comprehensive Gene Ontology (GO) enrichment analyses were performed to evaluate the biological functions of all identified DEGs. The GO analysis, encompassing Biological Processes (BP), Cellular Component (CC), and Molecular Function (MF), is presented in Fig. 4G. Within BP, the most...
7D). To better understand these downregulated proteins, we performed Gene Ontology (GO) biological process (BP) enrichment analysis and found that many members of this group mapped to RNA splicing machinery (Supplementary Fig. 11b). Prior reports have found that IpaH9.8 binds to and inhibits U2...
using |log2 fold change|≥ 1 and adjustedp-value < 0.05. The top 100 genes were then selected for enrichment analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis for DEGs within cell clusters were performed using Clusterprofiler (v4.8.3)...