LinRegPCR软件使用教程1、将CFX-96软件qRT-PCR的运行结果转化为LinRegPCR软件可以识别的Excel格式:主页面→tool→exportallsheetdatetoexcel(XXQuantitationAmplificationResults.xlsx为将要用到的数据)。2、打开上述Excel文件XXQuantitationAmplificationResults.xlsx(不要关闭该Excel,最终的计算结果要保存在此Excel工作薄中!)...
2. Introduction to the LinRegPCR program ...6 2.1 Definition of terms used in this manual and in the program...6 2.2 Basic procedure for using the program...7 2.3 Exporting raw data from the
Ct= -k lgX0+ b（线性方程）用这个方程可以通过Ct值算出样品的起始浓度 斜率=-1/lg(1+e) 扩增效率E=1, 斜率=-3.32 扩增效率范围：90%-110% 斜率范围：-3.1和-3.59之间 △△Ct这是相对荧光定量的一个计算公式的简化形式，用于比较不同样品之间的差。
The LinRegPCR web application provides visualization and analysis of a single qPCR run. The user interface displays the analysis results on the amplification curve analysis and melting curve analysis in tables and graphs in which deviant reactions are highlighted. The annotated results in the tables ...
From the menu select: Quantification - Export - Noise band, name the file you want to export (.TXT extension) and save it.7. Exit the LC program.Ruijter, J MRamakers, CRuijter J and Ramakers C. 2003. LinRegPCR analysis of real-time quantitative PCR data. Manual under construction....
LinRegPCRRDMLqPCRPCRAmplification curveMelting curveThe analyses of amplification and melting curves have been shown to provide valuable information on the quality of the individual reactions in quantitative PCR (qPCR) experiments and to result in more reliable and reproducible quantitative results.#The ...