Most qPCR machine software does not extract all relevant information that is present in the amplification curves. Because reaching the quantification threshold is often enough to consider a reaction to be positive, this also results in the assignment of a Cq value to a low-quality reaction. Althou...
4.1 LightCycler (Roche)To export the data set from a LC run, start the LC software program.1. Click on Data Analysis and open the file you want to export.2. (LC version 3.0) Click on Select-a-program (upper left corner) and choose `Amplification'3. Click the Quantification button.4....