aTo establish a link between SHMT alleles and soybean resistance to SCN, we scored 81 soybean lines representing 90% of the sequence diversity in soybean13 for SCN female index and then determined their SNP-based SHMT haplotype (Supplementary Table 1). The SHMT gene was fully sequenced from 28...
Erasp mutant alleles were generated via the CRISPR–Cas9 system. Two gRNA sequences were used to target Cas9 to the Erasp coding region. The targeting gRNA sequences used against the coding region of Erasp were gRNA1: 5'-AAAACGATCGACTGTCAGCTCGG-3'; gRNA2: 5'-CATTTTGCGAGACGGTATCTTGG-3...
The PCH15 EC8–9 sequence contains most of the canonical motifs involved in calcium binding (Supplementary Fig. 2), which typically define three calcium-binding sites, labeled 1 to 3, with several conserved, negatively-charged residues coordinating cal- cium ions (Fig. 2a–c; XEXBASE and DYE...
However, if γ>0, data could still contain alleles not observed in the frequency database used, but such data could then indicate a true, but until now unobserved, genotype. Similarly, m is related to the drop-out rate. When m is large (say m=1000), km≈12, and a drop-out can ...
The retinoblastoma gene was first described in children with eye cancer. In these patients, both alleles of a gene are defective; the gene is located on the long arm of chromosome 13 and encodes a protein with a molecular mass of about 105 kDa (RB105). Later, mutated forms of this prote...
Such alleles tend to be maintained at an intermediate frequency in populations by way of the process known as balancing selection. Positive selection favors the fixation of beneficial mutations that lead to evolution of new traits. Therefore, a mutation is said to be adaptive if it performs a ...
The Allele Translations table provides a key or legend linking the newly "translated" genotype to the original size in base-pairs score. Future exports use identical "allele translation codes." New alleles identified after the first export will be added to the end of a marker's allele ...
DNA–protein crosslinks (DPCs) arise from enzymatic intermediates, metabolism or chemicals like chemotherapeutics. DPCs are highly cytotoxic as they impede DNA-based processes such as replication, which is counteracted through proteolysis-mediated DPC re
In general, largest effect sizes of qualifying variants on metabolites were observed for variants with lower minor allele frequency, and stop gain and splice alleles tended to have larger effects than nonsynonymous variants (Fig. 2). Across the 30 genes, 2272 out of a maximum of 4864 studied ...
近日,来自加州大学旧金山分校的Alexander Marson团队在Nature上发表题为Base-editing mutagenesis maps alleles to tune human T cell functions的文章。该研究利用新开发的碱基编辑系统对原代T细胞的等位基因的功能进行筛选,为免疫疗法提供新工具。 为了能够在人原代T细胞中使用高通量使用碱基编辑器,作者优化了腺嘌呤碱基编辑...