Employing a combination of reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) techniques, the complete coding sequence of cDNA for the equine SRY gene was determined. We also attempted to make clear whether the equine SRY gene transcript is expresse...
Saccharomyces cerevisiae mitochondrial glutaredoxin 5 (Grx5) is the archetypical member of a ubiquitous class of monothiol glutaredoxins with a strictly conserved CGFS active-site sequence that has been shown to function in biological [Fe2S2](2+) cluster trafficking. In this work, we show that re...
Among them, OPTN exhibits the highest sequence homology to NEMO12. Unlike NEMO, OPTN does not interact with IKKα and IKKβ13; however, OPTN suppresses NF-κB activity by competing with NEMO function14. Furthermore, OPTN binds IKK subfamily protein kinases, such as TANK-binding kinase 1 (...
Oligonucleotides corresponding to the amino-acid sequence of synthetic peptide SP32 were cloned and over-expressed in Escherichia coli as a recombinant glutathione- S -transferase (GST)SP32 fusion protein. In ELISAs, this protein did not react with human anti-EV71 IgG antibodies, but there was ...
Although pro-NT binding to sortilin is partly inhibited by neurotensin (25) the size of the pro-NT pro-domain (∼100–110 amino acids) likely precludes entry of the entire domain into the tunnel. We therefore set out to study if a linear surface exposed amino acid sequence in sortilin ...
Sequence-dependent reactivity of linear DNA to chemical cleavage by Cu(II):thiol combinations including cysteine or glutathione The chemical cleavage of linear DNA by Cu(II):thiol combinations was investigated using end-labelled double-stranded restriction fragments. Single-strand c... DCA John,KT ...
Sequence and epitope analysis of surface proteins of avian influenza H5N1 viruses from Asian patients Avian influenza virus strain H5N1 is a highly patho- genic type A influenza virus that has caused several outbreaks of severe poultry plagues in the past. ... G Li,S Tao,X Wang - 《科学通报...
However, the specific role of Orc2-NTD (1-235) in pre-RC assembly is largely unknown. Sequence analysis using DISOPRED predicts that Orc2-NTD (1-235) is an extended intrinsically disorder region (IDR), in agreement with its flexible feature in the ORC-DNA structure (Fig. 1a, b) and ...
This AEP releases the C-terminal propeptide of kB1, but it does not mediate the N-terminal processing event, which must occur first if efficient cyclization is to take place. Moreover, its specificity for model peptides mirrors the sequence requirements for cyclization of kB1 in transgenic ...
Figure 1. Arp2/3 Complex Preferentially Nucleates Linear Actin Filaments in the Presence of Both Dip1 and Wsp1 (A) TIRF microscopy images of actin polymerization assays containing 150 nM glutathione S-transferase (GST)-Wsp1-VCA, 1.5 μM 33% Oregon-Green-labeled actin, 50 nM SpArp2/3 comp...