descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques Poor access to suitable light-sheet microscopes remains a big obstacle for many end-users in biomedical research. Here, the authors present descSPIM, a low-cost, low-expertise light-sheet ...
Light-sheet microscopy uses a thin plane of light to illuminate a sample orthogonally to the detection objective such that the axial resolution of the microscope is determined wholly or partly by the thickness of the light sheet. It allows fast volumetric imaging with reduced sample irradiation comp...
resulting in the illuminated specimen appearing dark against a bright background. The two lenses present in a compound microscope are the ocular lens in the eyepiece and the
Fluorescence Microscope: This type of microscope uses a light source that excites fluorescent molecules within the sample, causing them to emit light of a different color. It is used to observe samples that have been labeled with fluorescent dyes or antibodies, such as cells and tissues. Confocal...
The two transparent optical media that form an interface are distinguished from one another by a constant called the index of refraction, generally labeled with the symbol n. The index of refraction for any transparent optical medium is defined as the ratio of the speed of light in a vacuum ...
A stereomicroscope 21, known per se, is depicted only schematically, the microscope objective here being labeled 23 and the zoom systems for the left and right stereoscopic channels being labeled 22. The side view of stereomicroscope 21 means that only one of the two stereo channels, and ...
The waveforms for spatial axes (X, Y) can be achieved by sending triangular waveforms to a pair of galvo mirrors for laser positioning (labeled as GM2 and GM3 in Figure 3). To generate a triangular waveform in the frequency domain (Ω), a stable and high-speed frequency tuning is ...
Adult fireflies exhibit unique flashing courtship signals, emitted by specialized light organs, which develop mostly independently from larval light organs during the pupal stage. The mechanisms of adult light organ development have not been thoroughly s
Samples were visualized on an Olympus FV-1000 confocal microscope on an IX-81 inverted microscope. Experimental light exposures For shipboard light exposure experiments, a control group remained in the dark, while two experimental groups were exposed to different levels of fluorescent room light. Room...
(A) (Left panels) About 4−5 DN1ps lacking CRY protein expression, examples of which are marked with white arrows, were labeled with cytosolic-GFP and nls-GFP driven by the intersectional driver Clk4.1M-Gal4 + cry-Gal80. Clk4.1M-Gal4 alone drove GFP expression in about 10 of the ...