LIGAND BOUND MBP MEMBRANES, USES AND METHOD OF MANUFACTURINGCompositions and methods are described for self-assembled polymer materials having at least one of macro, meso, or micro pores.Rachel M. DorinSpencer RobbinsMark Hurwitz
the ligand-bound holo conformation is unknown, and the most readily available protein structure is the one predicted by AlphaFold, which often significantly differs from the holo conformation. Given that the AlphaFold-predicted structure often already complies with most chemical...
et al. A bioinspired approach for controlling accessibility in calix[4]arene-bound metal cluster catalysts. Nat. Chem. 2, 1062–1068 (2010). Article Google Scholar Nigra, M. M. et al. Accessible gold clusters using calix[4]arene N-heterocyclic carbene and phosphine ligands. Dalton Trans. ...
Although lacking the apo crystal structure, analysis of the tmCBP ligand bound state suggests the binding site may be pre-ordered for binding of either laminarins or cellodextrins. Essentially no structural differences are observed among the amino acids in the ligand recognition sphere of either ...
Many types of cells can sense external ligand concentrations with cell-surface receptors at extremely high accuracy. Interestingly, ligand-bound receptors are often internalized, a process also known as receptor-mediated endocytosis. While internalization is involved in a vast number of important functions...
However, treatment of T cells with inhibitors of γ-secretase causes an accumulation of a membrane-bound Notch1 stable intermediate [23]. In Notch1lbd, removal of aa 290–481 from the Notch1 ECD does not inhibit Notch1 synthesis, trafficking or stability at the plasma membrane. Nevertheless,...
Conformational plasticity of ligand-bound and ternary GPCR complexes studied by 19F NMR of the β1-adrenergic receptor G-protein-coupled receptors (GPCRs) are allosteric signaling proteins that transmit an extracellular stimulus across the cell membrane. UsingF NMR and site... JN Frei,RW Broadhurst...
Thus, although 9EG7 may reliably detect the ligand-bound conformation of {beta}1 integrins, its expression does not always correlate with integrin "activation." Finally, mouse/chicken {beta}1 chimeric molecules were used to map the 9EG7 epitope to {beta}1 residues 495-602 within the cysteine...
in co-IP assays. While addition of GDP increased XLG2–AGB1 interaction by ~70% compared to control, addition of GTP-γ-S diminished the interaction to 34% of the control (Fig.1a). These data are consistent with the expected role of GDP- and GTP-bound XLG2 in XLG2-Gβγ activation...
Taken together, these techniques allow for a high-throughput characterization of the mechanical stability of a library of receptors - without and with a ligand bound - in a single automated AFM-SMFS experiment. Our study focuses on all seven cohesins from A. cellulolyticus’ scaffoldin ScaA (...