VII. Selection of subpopulations using lectin-coated magnetic beads. Vet Immunol Immunopathol. 1995;44:181–95.Higgins D A,Ko W K W.Duck lymphocytes.Ⅶ.Selection of subpopulations using lectin-coated magnetic beads[J].Vet Immunol ImmunopathOL,1995,44:181-195....
We present an extension of this technology, using antibody- or lectin-coated magnetic beads (Dynabeads) to recover target nematodes from mixtures of specimens. Lectins and antisera that bound specifically and reproducibly to the whole surface of second stage juveniles of Globodera rostochiensis and ...
10 Screening for anti-LSECtin antibodies was done by enzyme-linked immunosorbent assay using 96-well plates coated with HIS-LSECtin or HIS-β-gal (negative control) fusion proteins. Final selection of the selected hybridoma (SOTO1) was done by immunofluorescence on HEK293T transiently ...
TFMI-3_HA was incubated with plasma and captured by anti-HA antibody coated magnetic beads. Competitive elutions were performed in two consecutive steps by incubating the beads with large excess of HA peptides for 5 and then for 90 minutes. Proteins bound to the inhibitor were identified ...
For ELISA-based binding assay, test antigens were coated in a concentration of 20 µg/ml in PBS on medium-binding half area 96-well microtiter plates (Greiner Bio-One, Kremsmünster, Austria) at 4 °C overnight. Wells were washed with 0.05% Tween 20 in PBS and blocked with 1% ...
Matrix: MagneZOOM™ (Paramagnetic Silica Coated Beads) Particle size range: 2-5 µm Matrix attachment: Amide Binding Capacity: 1-2 mg/ ml Inhibiting/Eluting Sugar: Chitin hydrolysate or GlcNAc with acid or salt Carbohydrate for Elution: .2M Chitin hydrolysate or GlcNAc with acid or salt ...
we developed a semi-automated high-throughput glycoprotein biomarker discovery platform termed lectin magnetic bead array-coupled tandem mass spectrometry (LeMBA-MS) which includes (i) effective single-step serum glycoprotein isolation using a panel of 20 individual lectin-coated magnetic beads in micropl...
In this study, several BRGs, including ica, fnbA, and fnbB, were rapidly detected (within 1 h) with a new integrated microfluidic system. Mannose-binding lectin (MBL)-coated magnetic beads were used to isolate these bacteria, and on-chip nucleic acid amplification (polymerase chain reaction, ...
In magnetic tweezer measurements using fibronectin-coated paramagnetic beads, the stiffness of keratinocytes was higher than of myoblasts. Interestingly, cell stiffness, adhesion strength, and cytoskeletal dynamics were strikingly altered in plectin-deficient compared to wild-type myoblasts, whereas smaller ...
Non-DC-lineage cells were coated with mAb (KT3-1.1, anti-CD3; T24/31.7, anti-Thy1; TER119, anti-erythrocytes; ID3, anti-CD19; and 1A8, anti-Ly6G) then removed using anti–rat Ig magnetic beads (Biomag beads, Qiagen, Victoria, Australia). Blood DCs were enriched by removing red ...