Objective To establish an LCMV-CL13 chronic infection mouse model and analyze its use for B cell somatic hypermutation research. Methods C57BL/ 6N mice were inoculated with 2×106 plaque-forming units of LCMV- CL13 virus via tail vein injection. The tissue viral load was then detected by qua...
The immunosuppressive LCMV isolates WE54, LCMV Cl13, and Traub efficiently target antigen presenting cells (dendritic cells) in the spleen and perturb their ability to present antigen to T cells and B cells, resulting in a generalized immunosuppression of the host allowing viral persistence. These ...
Furthermore, CD11b+DCs generated during CL13 infection could not induce effective CD8+T cells specific to the antigens of newly invading pathogens. Our findings demonstrate that DCs generated from the BM during chronic viral infection cannot activate fully functional effector CD8+T cells specific to...
LCMV (lymphocytic choriomeningitis virus) infection in mice is a well-established model for chronic infection, leading to T-cell exhaustion following chronic infection in mice1. Compared to effector CD8 T cells generated following an acute infection, exhausted CD8 T cells have impaired effector functio...
LCMV cl-13 is an established model of a persistent virus infection (reviews; (Asano and Ahmed, 1995, Oldstone, 2002, Oldstone, 2006, Oldstone et al., 1985, Yi et al., 2010)). The LCMV model has led to the development of experimental therapies to treat persistent infections (Finnefroc...