LC-ESI-HRMS在化合物结构研究中的应用
其中,液相色谱联用质谱技术(LC-MS)因其高灵敏度、高分辨率和高选择性等特点,已经成为化合物结构鉴定和定量分析的重要工具。在这些技术中,液相色谱-电喷雾离子化高分辨质谱(LC-ESI-HRMS)被广泛应用于化合物结构研究中,尤其是在天然产物的分离和鉴定方面,具有很高的分辨率和灵敏度,可以为天然产物的鉴定和研究提供重要...
尽管LC/ESI/HRMS是目前首选的分析技术,但定量本身就受到限制。ESI中的电离效率(IE)在很大程度上取决于化合物的物理化学性质,例如极性、酸碱特性、分子体积、所用洗脱液的性质和电离源几何结构。因此,化合物的IE可以相差几个数量级。因此,从LC/ESI/HRMS分析获得的信号并不能指示样品中化合物的绝对浓度。定量信息可以...
erucoides (L.) DC. (Brassicaceae) collected in Sicily (Italy). RESULTS Liquid chromatography coupled with electrospray ionization and high‐resolution mass spectrometry (LC‐ESI/HRMS) analysis of the ethanol extract revealed the presence of 42 compounds – glucosinolates, hydroxycinnamic acids, ...
Development of a metabonomic approach based on LC-ESI-HRMS measurements for profiling of metabolic changes induced by recombinant equine growth hormone in horse urine. [Research Support, Non-U.S. Gov’t Validation Studies]. Analytical and Bioanalytical Chemistry, 394 (8), 2119–2128. : 10....
LC-ESI-HRMS在化合物结构研究中的应用
To our knowledge, this is the first application of nanoLC/ESI + -HRMS 3 Orbitrap methodology to quantitative analysis of DNA adducts in vivo.doi:10.1007/s13361-014-0916-xDewakar SangarajuPeter W VillaltaSusith WickramaratneJames Swenberg...
Bhandi;M;M;Borkar;R;M;Shankar;G;et;al.Identification and characterization of stressed degradation products of Rabeprazole using LC-ESI/MS/MS and1H NMR experiments:in vitro toxicity evaluation of major degradation products.RSC Advances.0
Influence of Major Polyphenols on the Anti- Candida Activity of Eugenia uniflora Leaves: Isolation, LC-ESI-HRMS/MS Characterization and In Vitro Evaluationdoi:10.3390/molecules29122761POLYPHENOLSGALLIC acidEUGENIAELLAGIC acidPHYTOCHEMICALSCANDIDAPLANT polyphenols...
Jacob CC, Dervilly-Pinel G, Biancotto G, Monteau F, Le Bizec B (2015) Global urine fingerprinting by LC-ESI(+)-HRMS for better characterization of metabolic pathway disruption upon anabolic practices in bovine. Metabolomics 11(1):184-197...