MALDI imaging also detected differential analyte distributions of lactosylceramide species and gangliosides in the 4L/PS-NA brain, which was validated by LC-ESI-MS/MS. Immunohistochemistry revealed regional inflammation, altered autophagy, and defective protein degradation correlating with regions of GSL...
CeramideDihydroceramideLC/MS/MSQuantitationSerumPregnancy baselineCeramides and dihydroceramides are sphingolipids that present in abundance at the cellular membrane of eukaryotes. Although their metabolic dysregulation has been implicated in many diseases, our knowledge about circulating ceramide changes during ...
The strategy involved synthesis of ceramide [NP]-D3-18 (deuterated CER [NP]) to distinguish it from the endogenous CER [NP] in MS on weight basis. The method was linear over 10–800 ng/ml and sensitive with a limit of detection (LOD) and limit of quantification (LOQ) of 3 and 10...
CeramidesNude mouse skinA sensitive and accurate normal-phase liquid chromatography and atmospheric pressure chemical ionization mass spectrometry (LC–APCI–MS) method for determining the standard ceramide [NS] (Cer[NS]) was developed and validated so as to improve the traditional thin-layer ...