Recently, a cloning vector was developed which combines the ease of creating libraries in a lambda phage vector with the power of screening libraries using an M13 phagemid display system (27). The coliphage fd,
The vector, λglALO·λB, was constructed by crossing the Oam29, Aama1 and Lam439 mutations into λgt·λB. The mutation blocking phage DNA replication, Oam29, is suppressed by suII+ or suIII+. The head gene mutation, Aama1, is suppressed by suIII+ but not by suII+ and the ...
专业医学词典 λ嗜菌体媒介 与"lambda phage vector"相近的词条 ...
LAMBDA phage vector cloning kit Boiling point Melting point Density MSDS Formula Use,LAMBDA phage vector cloning kit 融点、価格、蒸気圧、沸点、毒性、比重、沸点、密度、分子式、分子量、物理的な性質、毒性.
1- Coliphage lambda DNA is a widely used vector for recombinant DNA. The middle third of its 48,000 bp contains no genes required for lytic growth and is, therefore, replaceable. The usual recombinant lambda DNA contains 80% lambda vector DNA and 20% insert, as compared to a usual cosmid...
Finally, we incorporate the lambda phage recombination sites directly into an HSV-2 BAC vector for direct recombination of gene cassettes using the phage lambda clonase-driven recombination reaction. Together, these improvements to the techniques of HSV BAC mutagenesis will facilitate the construction of...
STORAGE CONDITIONS Lambda ZAP-CMV XR Vector: –20°C Test Insert: –20°C Helper Phage: –80°C Bacterial Glycerol Stocks: –80°C Packaging Extracts: –80°C NOTICE TO PURCHASER The use of the CMV Promoter is covered under U.S. Patent Nos. 5,168,062 and 5,385,839 owned by the ...
vector relies on the presence ofcossites in the vector. During the ligation reaction, long concatamers are formed which are recognized by the packaging proteins and a certain length of DNA is packaged into the phage head. This size selection is a big help because it reduces the total number...
The system developed for making a lambda DNA particle vaccine for PCV2 expression in pigs is as follows. The phage vector was derived from a non-commercial vector, lambda Δb189 ΔKH54 BW1 Δnin5 QSR80. This vector forms large plaques, gives high titers with LB medium into 1011pfu per ...
1) lambda phage vector λ噬菌体载体2) λ phage λ噬菌体 1. To generate NPM gene knockout mice and study its biological function in vivo ,we screened the λ phage genomic library derived from 129S1 mice with mouse NPM cDNA probe. 为获得包含NPM基因组全长序列的噬菌体克隆,以小鼠NPMcDNA...