Cosmids can be packaged in lambda phage particles for infection into E. coli; this permits cloning of larger DNA fragments (up to 45kb) than can be introduced into bacterial hosts in plasmid vectors. Crossing over The breaking during meiosis of one maternal and one paternal chromosome, the...
Cosmids can be packaged in lambda phage particles for infection into E. coli; this permits cloning of larger DNA fragments (up to 45kb) than can be introduced into bacterial hosts in plasmid vectors. Crossing over The breaking during meiosis of one maternal and one paternal chromosome, the...
During embryogenesis, the genome shifts from transcriptionally quiescent to extensively active in a process known as Zygotic Genome Activation (ZGA). InDrosophila, the pioneer factor Zelda is known to be essential for the progression of development; still, it regulates the activation of only a small...
A phage vector lambda SK9 for construction of genome libraries along identification sections of restrictase BamI and Eco RI has the size of 47.7 k.b.p.; the vector capacity is from 4.5 to 19.0 k.b.p.; the vector consists of lambda EMBL 3 connected to phasmid SK2DELTASR which is ...
In the early 80s, Sanger successfully completed a whole genome sequencing of the lambda phage by using the shotgun method, and the method was successfully applied to the larger virus DNA, the organelle DNA, and the sequencing of the bacterial genome DNA. Shotgun sequencing is a classic strateg...
As a control for bisulfite conversion, 10 ng of unmethylated lambda phage DNA (#D1501, Promega) was added to the 1 μg of input DNA. Libraries were made with an in-house Illumina sequencer-compatible protocol. The extracted DNA was fragmented by S-series Focused-ultrasonicator (Covaris,...
MinION® tests were performed by all early access members, first on the phage lambda genome. Three recent publications of these studies [8-10] showed the production of long reads with an average size of 5,000 and 5,500 bp, respectively. These primary studies point to a high error rate ...
Meyer JR, Dobias DT, Weitz JS, Barrick JE, Quick RT, Lenski RE (2012) Repeatability and contingency in the evolution of a key innovation in phage lambda. Science 335(6067):428–432. https://doi.org/10.1126/science.1214449 Article CAS PubMed PubMed Central Google Scholar Bradley DE (196...
Lysates of androgenetic blastocysts were spiked with 0.1 ng lambda phage DNA and subjected to WGBS library construction according to the PBAT protocol for single-read sequencing47. DNA from 20–30 pooled 2C embryos per replicate was purified and spiked with 1% unmethylated lambda phage DNA, ...
The localization of genes identified from pan-genome analysis on the chromosome ofB. anthracisAmes. Red lines indicate the genes identified in this study, while blue boxes represent the lambda prophage regions described in a previous study [27]. An additional new region was identified between the ...