We also performed a chromatin immunoprecipitation sequencing (ChIP-seq) analysis using a lactylation-specific antibody and found that the histones H4K12la and H3K14la were enriched in the promoter and exon regions of T. gondii associated with microtubule-based movement and cell invasion. We ...
Glypican-3-specific antibody drug conjugates targeting hepatocellular carcinoma. Hepatology. 2019;70(2):563–76. Article CAS PubMed Google Scholar Yao G, Yang Z. Glypican-3 knockdown inhibits the cell growth, stemness, and glycolysis development of hepatocellular carcinoma cells under hypoxic ...
(f) The two types of the cells were treated as described above, and the expression of the lactylated proteins was detected with a pan-lactyl lysine antibody. The expression of β-actin served as an internal control Full size image Hypoxia aggravated LPS-induced neuroinflammation in a lactate-...
Consistent with the proteomics analysis, using this antibody, we detected α-MHC K1897 lactylation in control mice (Fig. 1k) and its lactylation level was decreased in mice with heart failure (Fig. 1l). Importantly, we found that in human pathological cardiac samples, the concentration of ...
(Supplementary Fig.4b–d), which implied the activation of Jurkat cells in the co-culture system was mostly due to PD-L1 knock-out. Meanwhile, blockage of PD-1/PD-L1 interaction by treating Jurkat cells with PD-1 neutralizing antibody toripalimab also restored Jurkat cell activation (Fig....
Using a specific antibody against lactylated K91, enhanced TFEB lactylation was observed in clinical human pancreatic cancer samples. Our results suggest that lactylation is a novel mode of TFEB regulation and that lactylation of TFEB may be associated with high levels of autophagy in rapidly ...
We used rabbit polyclonal anti-lactyllysine antibody (PTM-1401, PTM Biolabs), rabbit monoclonal anti-lactyllysine antibody (PTM-1401RM, PTM Biolabs), mouse monoclonal anti-CaM Kinase II α subunit antibody (05-532, Merck), mouse monoclonal anti-GAD67 antibody (MAB5406, Millipore), mouse ...
5E and F). Furthermore, the anti-c-Myc antibody increased the enrichment of the PKM2 promoter in PANC-1 and HPAF-II cells (Fig. 5G). In summary, RHOF promotes PKM2 expression by upregulating c-Myc expression. Fig. 5 GSEA analysis indicates RHOF activates EMT, glycolysis, and c-Myc ...
incubation with the corresponding primary antibodies overnight at 4 ℃. The cells were then incubated with corresponding fluorescent secondary antibody for 1 h at room temperature in the dark, and the DAPI staining reagent was stained for 10 min in the dark. Finally, the coverslips were placed ...
gondii using western blotting and the indirect fluorescent antibody test (IFA), respectively. Results: A total of 983 Kla sites occurring on 523 lactylated proteins were identified in the total protein extracted from Toxoplasma tachyzoites, the acute toxoplasmosis-causing stage. Bioinformatics analysis...