Lactate dehydrogenase 5 (LDH-5) is one of the major isoenzymes catalyzing the biochemical process of pyruvate to lactate. The purpose of this study was to investigate the expression of serum LDH-5 and test whether this enzyme is regulated by tumor hypoxia and represents a prognostic marker in...
After a period of incubation, the medium was collected and frozen at −80 °C to deactivatel-lactate dehydrogenase prior to [lactate] measurements on a YSI 2500 Biochemistry Analyzer (525000, Xylem) or ABX Pentra C400 analyzer (Horiba). Western blotting Samples were sonicated, denatured by ...
First, we investigated whether concomitant measurement of lactate secretion would alter the assessed cellular functions, the frequency of IgG-SCs, IgG secretion rate and affinity. For this purpose, these parameters were measured in the presence and absence of the lactate bioassay. In short, we did ...
Lactate-grown cells utilize an alternative respiration pathway involving an external respiratory protein, NADH-dehydrogenase (alternate complex 1), which is unable to translocate protons to the mitochondria, thereby affecting energy production. In response, these cells up-regulate cytochrome Cyb2, a ...
The decrease in l(+)-lactate in the previous studies coincided with an increase in d(−)-lactate, concluded to be caused by heterofermentative NSLAB that contained l(+)- and d(−)-lactate dehydrogenase enzymes able to racemize l(+)-lactate to d(−)-lactate. In the present study,...
The purpose of the invention is to use both natural and synthetic minerals of the group of the zeolites for absorption, adsorption, and neutralization, as well as for lowering the proportion of lactates or ammonium in humans and animals. The zeolites have been crushed in conventional mineral or...
Lactate is derived from the glycolytic reduction of pyruvate, a reversible reaction that is catalyzed by lactate dehydrogenase (LDH). The relative amount of metabolism via LDH in the forward (pyruvate + NADH + H+→ lactate + NAD+) and reverse directions (lactate + NAD+→ pyruvate + NADH +...
we developed a screening procedure based on the capacity of cancer cells to release L-lactate to gain insights on the development of mitochondrial complex I inhibitors. For this purpose, we synthesized derivatives of carboxyamidotriazole, a compound previously described as a potential OXPHOS inhibitor...
Data from the impact of paediatric atopic dermatitis on parental rest are scarce. The purpose of this study was to examine the consequences of paediatric atopic dermatitis from the quality of parents’ rest. This cross-sectional study included moms and dads of clients with atopic dermatitis and pa...
In a previous study, immuno-polymerase chain reaction (PCR) was shown to provide highly sensitive detection of Plasmodium falciparum lactate dehydrogenase (PfLDH) in monoclonal antibody (mAb) sandwich assays. Here, we show comparably high immunoassay sensitivity by inductively coupled plasma mass ...