LactateAssayKit40MAK06441-TechnicalBulletinSigma-Aldrich 系统标签: lactateassaykitbulletinaldrichnmole LactateAssayKitCatalogNumberMAK064StorageTemperature–20°CTECHNICALBULLETINProductDescriptionL(+)-LactateisametaboliccompoundformedinanimalsbytheactionoftheenzymeLactateDehydrogenase(LDH).Lactateisproducedinproliferating...
After washing with PBS, the nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma‒Aldrich, St. Louis, MO, USA), and the sections were mounted and observed via confocal microscopy (X-Light V3; CrestOptics, Rome, Italy)15. Western blotting analysis Lung tissue samples or ...
sodium D (−) lactate, D-2-hydroxyisovaleric acid, L-2-hydroxyisovaleric acid, L-2-hydroxyisocaproic acid, (+)-O,O-diacetyl-L-tartaric anhydride (DATAN), and ammonium formate were obtained from Sigma Aldrich (St. Louis, MO, USA). [13C3]-Sodium L (+) lactate was obtained from ...
Nicholas J., KenyonJennifer, M. BrattJoyce, LeeJuntao, LuoLisa M., FranziAmir, A. ZekiKit S., Lam
either CD14+ magnetic cell sorting (Miltenyi Biotech, Bergisch Gladbach, Germany) or by allowing mononuclear cells to adhere to plates (1 × 106 cells per mL; Falcon; Becton Dickinson, Franklin Lakes, NJ, USA) for 1 h in RPMI 1640 medium (Sigma-Aldrich, St. Louis, MO, USA) at +37 ...
Whole-cell or tumor lysates were prepared using RIPA buffer (Sigma Aldrich, St. Louis, MO, USA, R2078) with protease inhibitor cocktail set III (Millipore, Burlington MA, USA, 539134) at 4C and quantified using a PierceTMBCA Protein Assy Kit (ThermoFisher, #23225) before blotting. Lysate...
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All other reagents were from Sigma-Aldrich (MO, USA) unless stated otherwise. Cell culture Panc-1 cells from ATCC were cultured in Dulbecco’s modified Eagle’s medium containing 4.5 g/L glucose supplemented with 10 % fetal bovine serum and penicillin-streptomycin. Panc-1 cells were treated ...