D-Lactate production in mammals, mainly due to the glyoxalase pathway, is extremely low, with normal serum concentrations in the nano to micromolar range. Typically, elevated D-lactate levels which can rise to millimolar levels, are due to bacterial infection or short bowel syndrome in humans. ...
Mongersun, Amy, et al. (2016). Droplet Microfluidic Platform for the Determination of Single-Cell Lactate Release. Analytical chemistry 88.6: 3257-3263.Assay: L-Lactate in unknown cancer cells. Saville, Renee M., et al (2016). Compositions and methods for biological production of lactate from...
Lactate production assay Lactate production was detected using a Lactate assay kit (BioVision, Inc., Milpitas, CA, USA). Results were normalized to the quantity of total protein in the control cells. LDH activity assay Total LDH activity in cell lysates was examined using the LDH cytotoxicity a...
Senadheera, D., et al. (2009). Inactivation of VicK affects acid production and acid survival of Streptococcus mutans. J Bacteriol 191(20):6415-24.Assay: L-Lactate in Streptococcus mutans bacteria. Weber, J., et al. (2009). Hyperalgesia induced by oral stavudine administration to rats doe...
This Amplite® Lactate Dehydrogenase Assay Kit provides an absorption-based method for detecting D-lactate dehydrogenase (D-LDH) in biological samples such as serum, plasma, urine, as well as in cell culture samples. In the enzyme coupled assay, LDH is proportionally related to the ...
Rubicon enhances VPS34 activity and maintains PI3P on LAPo- somes while also stabilizing the NOX2 complex for ROS production, both of which are essential for LAP progression19. To determine whe- ther RUBCN lactylation influences these processes, RUBCN-knockout THP-1 cells were reconstituted with...
for measurements in a direct respiratory chamber. The body weights of the steers were measured at the beginning and at the end of the experiment. Feces and urine were collected for 5 days after 1 day of adaptation to the chamber, methane production was measured for 2 days, and ruminal fluid...
In addition, gradients across the heart do not take myocardial production of lactate from e.g. glycogen into account [57]. Finally, while permeabilized fiber techniques are a well-validated approach for assessing mitochondrial respiratory capacity, they do not entirely replicate the natural ...
生物学论文:NAD-依赖型乳酸脱氢酶及其在α-羟基羧酸生产中的应用 NAD-dependent lactate dehydrogenase and its application in production of α-hydroxy carboxylic acids 热度: 乳酸脱氢酶的免疫调节机制 热度: 乳酸脱氢酶在免疫细胞代谢中的作用 热度: 相关推荐 1 人D-乳酸(D-Lactate)酶联免疫分析 试剂盒...
l-Lactate production was measured using the EnzyChrom l-Lactate Assay kit (BioAssay Systems). The protocol for the analysis of interaction between recombinant NDRG3-GST protein and l-[14C]-lactate (PerkinElmer) is described in Extended Experimental Procedures. Statistical Analysis Statistical significan...