d, Metaprofile of Ago2 LACE-seq signals from control (ctrl) or Ago2−/− mouse oocytes around the identified peaks. e, An example of abolished Ago2 binding at the transcript Tuba1a in Ago2-null oocytes. f, The number of reads and the identified peaks by the LACE-seq protocol with...
(Supplementary Figure 1). Meta- genomic libraries of the Genomiphi-amplified 13C- Results labelled DNA from 'heavy' and 'light' fractions were Assessment of DMS uptake potentials in lake sediment sequenced using the Illumina HiSeq2000 technology and soil samples (GATC Biotech, Konstanz, Germany) ...
Sequencing was carried out on Illumina HiSeq 2000 using the protocol HCS 1.5.15.1 in single reads. For ribosome profiling with RiboLace, a total volume of lysate corresponding to 0.3 A.U.260nm was treated with 1.5 U of Artseq nuclease (Epicenter) or RNaseI for 45 min at 20°C. The ...
30. The system of claim 2, wherein the remote server is configured to have a different Internet Protocol address on at least one of the plurality of data channels, wherein the outgoing data packets on a data channel are addressed to an IP address of the server for the respective data cha...
Illumina library preparation & MiSeq sequencing Whole-genome-shotgun PCR-free libraries were constructed from 5 µg of gDNA with the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturer’s protocol. The libraries were quality controlled by analysi...
We sequenced the complete mitochondrial genome and the nuclear ribosomal gene segment of the avocado lace bug Pseudacysta perseae using a genome skimming approach on an Illumina Hiseq 2000 platform. Fifty-four additional heteropteran mitogenomes, including the one of the sycamore lace bug Corythu...
Illumina library preparation & MiSeq sequencing Whole-genome-shotgun PCR-free libraries were constructed from 5 µg of gDNA with the Nextera XT DNA Sample Preparation Kit (Illumina, San Diego, CA, USA) according to the manufacturer's protocol. The libraries were quality controlled by ...