In vitro analysis of the binding of AGAb and human polyclonal IgG to rat granulocytes showed a low level of binding with both agents. These results suggest that the primary mechanism of localization, by either antibody preparation in this model, is not antigen related. 111In-labelled nonspecific...
A new, simple procedure is described for the production of 5 nm colloidal gold/secondary antibody reagents. Utilizing them with antitubulin shows 1) that t... J Demey,M Moeremans,G Geuens,... - 《Cell Biology International Reports》 被引量: 604发表: 1981年 Application of the silver-gold...
Such tracing of proteins by means of labelling with fluorescent dyes has been a successful technique in recent years, particularly in examining antibody-antigen reactions 2 and has been used to follow the fate of labelled lipase added to growing tissue cultures 3 . The successful use of fluoro...
Based on these kinetic parameters, the amount of tumour-bound radiolabelled monoclonal antibody was calculated. This method permits estimates of both macrodosimetry and microdosimetry at the cellular level based on in vivo non-invasive measurement. 展开 关键词: Positron emission tomography Antibody ...
monoclonal antibody significantly inhibits the metastasis of breast cancer cells in mice. Similar results were obtained in other types of cancer (see reference [54] for review). Based on these findings, several groups exploited targeting of CXCR4 as a potential tool for the imaging of metastatic ...
1d). The fluorescence-tagging was also validated by immunoblotting for the presence of the mEmerald-CD81 (ca. 50 kDa) using a CD81 antibody (Fig. 1e) and by confocal microscopy (Fig. 1f). Triton X-100 and/or Proteinase K treatment of these fluorescent EVs indicated that the ...
Mite antigen was labelled with ^<125>I and used in double antibody assay of IgG and IgA antibodies in ten patients who were allergic to both of house dust and mite. IgE antibodies were estimated by RAST. Results are as follows. 1)Level of IgE, IgA and IgG antibodies were higher in 10...
Journal of Molecular BiologyShatsky IN, Mochalova LV, Kojouharova MS, Bogdanov AA, Vasiliev VD. Localization of the 3' end of Escherichia coli 16 S RNA by electron microscopy of antibody-labelled subunits. J Mol Biol. 1979 Oct 9; 133 (4):501–515....
For example, dinitrophenol has a commercially available fluorescent antibody that can be used for detection. The label (or label and linker construct) can be of a size or structure sufficient to act as a block to the incorporation of a further nucleotide onto the nucleotide of the invention. ...
McNiven for the MMR48 antibody; M. Fritz for the rhodamine-labelled tubulin; U. Euteneuer, H. Felgner, R. Gra¨f and S. Linder for advice; and S. Fuchs for technical assistance. This work was supported by a Graduiertenkolleg fellowship to U.H. and a grant from the DFG to M.S....