Efficient fluorescent labeling of DA neurons and their axons in AAV-LSL-mCherry injected DA neuron-specific Cre mice.Karsten, TillackHelia, AboutalebiEdgar, R. Kramer
Nanodiamond is a promising carbon nanomaterial developed for biomedical applications. Here, we show fluorescent nanodiamond (FND) with the biocompatible properties that can be used for the labeling and tracking of neuronal differentiation and neuron cells derived from embryonal carcinoma stem (ECS) cells...
Nascent RNA transcriptome-wide profiling neuron CuAAC UPRT 摘要 Transcriptome-wide expression profiling of neurons has provided important insights into the underlying molecular mechanisms and gene expression patterns that transpire during learning and memory formation. However, there is a paucity of tools ...
CRITICAL: To obtain optimal results, it is important to photo-convert a sufficient number of PA-GFP molecules in the neuron of interest, while preventing the neuron from being damaged. The laser power required for photo-labeling individual neurons varies from sample to sample and should be adjust...
Large injections in rostral DBB appeared to retrogradely label every DLSN neuron, while similar injections in caudal DBB only labeled neurons in restricted regions of the nucleus. A collection of small cells forming the ventricular border of caudal DLSN and a group of larger cells situated in ...
Tools for visualizing and manipulating protein dynamics in living cells are critical for understanding cellular function. Here we leverage recently available monoclonal antibody sequences to generate a set of affinity tags for labeling and manipulating A
A monoiodo derivative of rat atrial natriuretic factor (rANF) was shown to specifically bind to rat brain neurones in culture with low binding site capacity (10–20 fmoles per mg of protein) and high affinity (Kd = 50–100 pM). Several analogs of both rat and human ANF competed with ...
White arrowheads indicate the transfected neurons, yellow arrowhead indicates a neighboring untransfected neuron. Scale bars are 10 µm. e Quantification of A2-Fab-Halo (left panel) or A1 scFv-Halo (right panel) labeling in cells expressing saCas9 along with GluA2 or GluA1-targeting gRNA ...
Cancer cells inevitably interact with neighboring host tissue-resident cells during the process of metastatic colonization, establishing a metastatic niche to fuel their survival, growth, and invasion. However, the underlying mechanisms in the metastatic niche are yet to be fully elucidated owing to the...
9 RegisterLog in Sign up with one click: Facebook Twitter Google Share on Facebook affinity labeling Medical [ə′fin·əd·ē′lā·bə·liŋ] (biochemistry) A method for introducing a label into the active site of an enzyme by relying on the tight binding between the enzyme and...