For studying dynamic mitochondria membrane potential specifically, we recommend using JC-1 (cationic carbocyanine dye, Cat. No. T3168) or TMRM (tetramethyl rhodamine methyl ester, Cat. No. I34361) dyes. The MitoProbe JC-1 Assay Kit (Cat. No. M34152) contains the JC-1 dye in addition to...
For studying dynamic mitochondria membrane potential specifically, we recommend using JC-1 (cationic carbocyanine dye, Cat. No. T3168) or TMRM (tetramethyl rhodamine methyl ester, Cat. No. I34361) dyes. The MitoProbe JC-1 Assay Kit (Cat. No. M34152) contains the JC-1 dye in addition to...
Labeling Mitochondria with MitoTracker Dyes JC-1 (5,5',6,6'-tetrachloro1,1',3,3'-tetramethylbenzimidazolylcarbocyanine iodide) dye has been used to monitor mitochondrial potential. The monomeric form... B Chazotte - 《Cold Spring Harbor Protocols》 被引量: 104发表: 2011年 Allicin Induces p5...
2i). Finally, by immunofluorescence detection, we visualized the Ir1-catalyzed labeling signals inside cells, with the biotinylation signal precisely colocalized with the mitochondria marker (Fig. 2j, k). Statistical analysis further revealed high Pearson’s R values (0.87 for HeLa and 0.90 for ...
Adams JC (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40:1457–1463 Article CAS Google Scholar Rhee HW, Zou P, Udeshi ND, Martell JD, Mootha VK, Carr SA, Ting AY (2013) Proteomic mapping of mitochondria in living cell...
The functionality of the SpySystem in planta, combined with downstream applications, was proven. Using organelle-specific membrane anchor sequences to program the sub-cellular localization of the SpyTag peptide, we could tag the outer envelope of chloroplasts and mitochondria. By co-expression of a ...
The small probe volume, achieved by overlapping two laser beams, makes our technique compatible with microfluidics and capillary-based electrophoresis systems. ... N Shatirishvili - IOP Publishing Ltd 被引量: 0发表: 2024年 Label-free dynamic imaging of mitochondria and lysosomes within living cells...
To demonstrate the applicability of this new PL technology, we tested whether OMM-APEX2 and SOPP3-ERM (fused with endoplasmic reticulum (ER) membrane (ERM)-resident protein — SEC61B, facing cytosol) mediate PL at ER–mitochondria (Mito) contact sites (Fig.1d). Streptavidin blot also showed...
Rosamine & carbocyanine-based staining dyes MitoTracker Orange CMTMRos, Red CMXRos, Orange CM-H2TMRos, Red CM-H2XRos, Red FM, Green FM, & Deep Red FM enable mitochondria visualization with fluorescent imaging. These mitochondria dyes are specially packaged in vials of 50 μg each to create...
For studying dynamic mitochondria membrane potential specifically, we recommend using JC-1 (cationic carbocyanine dye, Cat. No. T3168) or TMRM (tetramethyl rhodamine methyl ester, Cat. No. I34361) dyes. The MitoProbe JC-1 Assay Kit (Cat. No. M34152) contains the JC-1 dye in addition to...