For most cells, the membrane potential of the mitochondria will be completely lost after 20 minutes of treatment with 10μM CCCP. After staining with JC-1, it should show green fluorescence; while normal cells should show red fluorescence after staining with JC-1. For specific cells, the ...
JC-1 is a novel cationic carbocyanine dye that accumulates in mitochondria. The dye exists as a monomer at low concentrations and yields green fluorescence, similar to fluorescein. At higher concentrations, the dye forms J-aggregates that ex
Probes for Mitochondria—Section 12.2 Slow-Response Probes—Section 22.3 Assays for Apoptosis and Autophagy—Section 15.5 常见问题解答 (FAQ) A membrane potential indicator selection guide can be found here (https://www.thermofisher.com/us/en/home/life-science/cell-analysis/cell-viability-and-regula...
JC-1 has advantages over other cationic dyes in that it enters the mitochondria and changes its fluorescent properties based on the aggregation of the probe. In healthy cells with high ΔΨM, JC-1 forms complexes known as J-aggregates with intense red fluorescence. However, in cells with ...
贝博® BBcellProbe® 线粒体膜电位检测试剂盒(JC-1)是利用JC-1探针检测线粒体膜电位变化的试剂盒,可以快速灵敏地检测细胞、组织或纯化的线粒体膜电位变化,可以用于早期的细胞凋亡检测。 本试剂盒染料JC-1是一种广泛用于检测线粒体膜电位(mitochondrial membrane potential)△Ψm的理想荧光探针。与其他的阳离子...
ab113850 uses tetraethylbenzimidazolylcarbocyanine iodide (JC-1), a cationic dye that accumulates in energized mitochondria. At low concentrations (due to low mitochondrial membrane potential) JC-1 is predominantly a monomer that yields green fluorescence with emission of 530±15nm. At high concentrati...
JC-1 has advantages over other cationic dyes in that it enters the mitochondria and changes its fluorescent properties based on the aggregation of the probe. In healthy cells with high ΔψM, JC-1 forms complexes known as J-aggregates with intense red fluorescence. However, in cells with ...
JC-1 mitochondrial membrane potential assay is a fluorescence-based method to track the behavior of mitochondria. Mitochondrial membrane potential (MMP) is an important parameter of mitochondrial function that has been used as an indicator of cell apoptosis. Several cell membrane permeable fluorescent dy...
JC-1通常由流式细胞仪中常见的488nm激光波长激发[1]。在暴露于小鼠L1210淋巴母细胞的JC-1 (2.5μM)中,可以检测到这些细胞中细胞质中JC-1单体和线粒体J聚集体的存在。通过使用JC-1 (1 μg/mL, 15 min)对线粒体进行荧光标记,揭示了它们在星形胶质细胞内分布不均,导致线粒体含量高低不一的区域[2]。JC...
线粒体荧光探针信息大全 (Probes for Mitochondria)包括各种常用探针,如JC-1,JC-9,TMRM,TMRE等 Mitochondria are found in eukaryotic cells, where they make up as much as 10% of the cell volume. They are pleomorphic organelles with structural variations depending on cell type, cell-cycle stage and ...