Plants cells have DNA that helps in making new cells, hence enhancing the growth of the plant. the DNA is enclosed within the nucleus, an enveloped membrane structure at the center of the cell. The plant cell also has several cell organelle structures performing a variety of functions to main...
a用标记的DNA探针杂交和放射自显影后形成的带纹分布应该是有区别的 The belt grain distribution which forms since the development should have the difference with the mark DNA probe hybrid and the emission[translate]
vacoules and chloroplast. As both animals and plants are eukaryotic organisms they also share many features within their cells, some of the organelles that they share include the nucleus, which contains the chromosomes and DNA; the ATP producing mitochondria; the protein processing golgi bodies and...
The Escherichia coli strain DH5α was used for cloning and amplification of the DNA whereas Escherichia coli BL21 (DE3) competent cells were used as host to express unlabeled (14N,12C) and labeled (15N,13C) WT horse cyt c and its mutant L94G as described previously16. The expression plas...
7C). These total amounts and proportions of plasmid DNA were chosen to maintain as much as possible the viability of the transfected cells and also enough of a fluorescence level of the double-labeled protein to perform the FRET measurements. Similar results were obtained when unlabeled wild-...
Fluorescence in situ hybridization (FISH) is used to visualize the distribution of DNA elements within a genome. Conventional methods for FISH take 1–2 days. Here, we developed a simplified, rapid FISH technique using pre-labeled oligonucleotide probes
DNA conjugate comprises: a tandemly linked DNA in which two or more DNAs encoding one or more types of target peptide fragments are linked in tandem; a DNA encoding a peptide fragment for concentration measurement conjugated with the 5′ end of the tandemly linked DNA; and a DNA encoding a...
Genomic DNA extraction Figure 1 Extraction of gDNA from cochlear turns. (a) Diagram of the approximate locations of guinea pig cochlear turns. Number 1 is the hook region and number 9 is the apical tip. (b) Example of a whole mount image of an immunolabeled cochlear half turn from the ...
Introduction In the central dogma of molecular biology, messenger RNA (mRNA) is the molecule linking the genetic code, DNA, with the cellular work force, proteins. mRNA is translated into proteins with the help of transfer RNA (tRNA) and ribosomal RNA (rRNA). In addition to these key ...
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