Label-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is technically challenging or even impossible. However, existing methods require the investigated
In the three root zones (LR/MR and LR/UR comparisons), we observed that impor- tant enzymatic steps in the glycolysis pathway and glucose metabolism were up-regulated. For exam- ple, phosphopyruvate hydratase (Zm00001d045431 _P002), glyceraldehyde-3-phosphate dehydroge- nase (Zm00001d051001...
The cell membrane is more than just a passive lipid bilayer barrier. Of special relevance, cell membrane proteins are an integral part of the cellular machinery concerning sensing and reacting to what surrounds the cell, through different processes such as signaling, transport and immune response. ...
As a validation of the enhanced sensitivity and specificity of our system, we show classification of white blood T-cells against colon cancer cells, as well as lipid accumulating algal strains for biofuel production. This system opens up a new path to data-driven phenotypic diagnosis and better ...
Glycolysis, Oxidative Pentose phosphate pathway (OPPP) and tricarboxylic acid cycle (TCA) provides carbon, reduced cofactors, and ATP for cellular functions [57,58]. In the three root zones (LR/MR and LR/UR comparisons), we observed that important enzymatic steps in the glycolysis pathway and ...
Drum drying is a common method in the food industry for pre-cooking grain-based products, such as gruel and porridge (Björck et al.,1984). The process is performed in several steps. First, the starch slurry is applied as a thin layer to the outer surface of the drum as the inside ...
effects of optical absorption and scattering. Photoacoustic imaging (PA) largely avoids optical scattering effects by utilizing acoustic rather than optical detection. However, although PA has been used to probe in vivo lipid content, current works have been limited to lipid-rich tissues such as ...
The majority of coherent Raman microscopy studies have been focused on exploring the metabolism of lipid droplets (LDs)14,18,19,20,21,22,23,24, while little has been directed toward understanding mitochondrial properties. In this study, we used CARS microscopy to image living cancer cells and ...
(r) represent the absorption and phase components of the object, respectively;rdenotes the spatial coordinates. While for ODT of 3D thick specimens, the object is characterized by the complex scattering potential\(O\left( {{{\mathbf{r}}} \right) = k_0^2\left[ {n\left( {{{\mathbf{r...
1. A method for determining the rate of biosynthesis of a biological molecule in an individual through the detection of a metabolic derivative of the biological molecule, the method comprising the steps of: a) administering a precursor molecule comprising an isotope label to the individual for a ...