(1) Fill in the Venn diagram below comparing the structures of the 2 nucleic acids. (2) Label all of the parts of the DNA molecule. (3) Circle a nucleotide on the diagram. (4) What three parts does the nucleotide contain? (5) Define antiparallel. Label the 3’ and 5’ ends. (6...
We selected an aptamer against a fluorogenic dye called Thioflavin T (ThT). Aptamers are single-stranded DNA that can bind a specific target. We selected the ThT aptamer using graphene oxide assisted SELEX and a low-cost Open qPCR instrument. We optimize
BmNPV proliferation changes upon differently treated BmNPV infected in larvae was determined by estimating viral DNA using specific primers of the VP39 gene by RT-qPCR [21]. There were three biological sample replicates, and each biological sample replicate included three technique replicates. The ...
MicroRNA (miRNA) was a promising class of cancer biomarkers. Here we developed a label-free method for sensitive measurement of let-7d miRNA based on multiple amplification techniques. The primer will bind to the duplex strand DNA that was formed by stem-loop template and target let-7d to in...
the combined use of bNAbs and CD8+T cells9. Moreover, in a recent study, HIV-1-specific T cells engineered to secrete bNAbs displayed superior activity in suppressing HIV-1 replication in vitro10. However, there has been no exploration of whether such a two-in-one approach could enhance ...
The signal amplification and background signal suppression are two main strategies for improving DNA fluorescence sensors sensitivity. Common methods based on signal amplification can effectively increase sensor sensitivity, including rolling circle replication (RCR) (Zhou et al. 2015), strand displacement ...
Basic configuration of the ptychographic microscope. A schematic diagram of the light path in the ptychographic microscope showing the laser, collimator, x,y scanning stage, objective lens, the aperture in the image plane and the 2 CCD cameras. ...
down-regulated and unique DAPs.b,c,dHeat map showing abundance profile of proteins in MR/UR, LR/MR, and LR/UR comparison. Proteins with high abundance (brown); proteins with low abundance (dark brown) of what is contained in the second panel.eVenn diagram showing unique and common DAPs...
range of the DNA machine is from 100 to 600 nM (R2= 0.99108) with a limit of detection (LOD) of 127.9 pM. A DNA machine circuit and AND-NOT-AND-OR logic gates were successfully constructed, and the strategy was used to detect ATP in human urine. With the advantage of target ...
Figure 1. Schematic diagram of an AMPA chip based on an oscillating micropillar array for cell separation. (a) General schematic of the AMPA chip. The process of capturing and separating cancer cells using acoustic streaming generated by microstructure vibration: (b) top view and (c) side view...