Cone-shaped dendrimer molecules have been immobilized on the gold surface at equidistance, 3.1 nm, from each other with a probe DNA molecule attached to the top of each dendrimer so that enough space would be s
Labeled-probe-assemblies, comprising a probe element, for binding to a target molecule, attached to a label-assembly further comprising a linear series of information encoded structures and means for spatially separating these structures, are presented together with methods for extending the label ...
The N protein, studied exclusively in living cells, binds the linear phage Mu chromosome non-covalently and holds it, with flanking E. coli DNA from the previous host cell, in a circular conformation during injection [9]. N is injected into the cell with the DNA molecule [[10], [11],...
During the whole procedure, L-glutamic acid acted not only as complexing and capping agent, but a bridging agent between DNA molecule and zinc selenide nanospheres. The ZnSe nanoshperes could be used for the detection of the DNA hybridization. DNA specific-sequence related to PAT gene in the ...
The meaning of PULSE-LABEL is to cause a pulse of a radiolabeled atom or substance to become incorporated into (as a molecule or cell component). How to use pulse-label in a sentence.
原理:Random decamers are annealed to a denatured template DNA molecule and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand (see Fig.1). ...
Kensuke Ogawa1, Noritada Kaji1,2, Mats Nilsson4, Taiga Ajiri5, Manabu Tokeshi2,6,Yasuhiro Horiike7 &Yoshinobu Baba1,2,8 Quantitative DNA amplification using fluorescence labeling has played an important role in the recent, rapid progress of basic medical and molecular biological...
原理:Random decamers are annealed to a denatured template DNA molecule and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand (see Fig.1). ...
Kovari, D.T.; Yan, Y.; Finzi, L.; Dunlap, D. 2018:Tethered Particle Motion: An Easy Technique for Probing DNA Topology and Interactions with Transcription FactorsMethods in Molecular Biology 1665: 317-340 Al Balushi, A.A.; Gordon, R. 2014:Label-Free Free-Solution Single-Molecule Protein...
Label-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is technically challenging or even impossible. However, existing methods require the investigated