Cone-shaped dendrimer molecules have been immobilized on the gold surface at equidistance, 3.1 nm, from each other with a probe DNA molecule attached to the top of each dendrimer so that enough space would be s
The meaning of PULSE-LABEL is to cause a pulse of a radiolabeled atom or substance to become incorporated into (as a molecule or cell component). How to use pulse-label in a sentence.
The N protein, studied exclusively in living cells, binds the linear phage Mu chromosome non-covalently and holds it, with flanking E. coli DNA from the previous host cell, in a circular conformation during injection [9]. N is injected into the cell with the DNA molecule [[10], [11],...
Labeled-probe-assemblies, comprising a probe element, for binding to a target molecule, attached to a label-assembly further comprising a linear series of information encoded structures and means for spatially separating these structures, are presented together with methods for extending the label ...
原理:Random decamers are annealed to a denatured template DNA molecule and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand (see Fig.1). ...
Label-free characterization of single biomolecules aims to complement fluorescence microscopy in situations where labeling compromises data interpretation, is technically challenging or even impossible. However, existing methods require the investigated
Kensuke Ogawa1, Noritada Kaji1,2, Mats Nilsson4, Taiga Ajiri5, Manabu Tokeshi2,6,Yasuhiro Horiike7 &Yoshinobu Baba1,2,8 Quantitative DNA amplification using fluorescence labeling has played an important role in the recent, rapid progress of basic medical and molecular biological...
原理:Random decamers are annealed to a denatured template DNA molecule and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand (see Fig.1). ...
During the whole procedure, L-glutamic acid acted not only as complexing and capping agent, but a bridging agent between DNA molecule and zinc selenide nanospheres. The ZnSe nanoshperes could be used for the detection of the DNA hybridization. DNA specific-sequence related to PAT gene in the ...
dideoxy sequencing is the Escherichia coli DNA-polymerase I large fragment ("Klenow"). Another polymerase used is AMV reverse transcriptase. SUMMARY OF THE INVENTION In one aspect the invention features a method for determining the nucleotide base sequence of a DNA molecule, comprising annealing the...