Label-free single-molecule DNA sequencingMicrofluidics can be used to trap a single DNA–enzyme complex in its native state, without immobilisation. These studies have usually been performed by immobilising either the enzyme or the DNA on ...Enzymes...
c) and the optical properties of the molecule and the nanochannel, defined by the total electric dipole polarizability (αt)24. The αt can be determined by the respective electric dipole polarizabilities of the biomolecule, αm, and of the nanochannel, ...
We report preparation of a novel platform for effective DNA hybridization and its application to the detection of single mismatched DNA. Cone-shaped dendrimer molecules have been immobilized on the gold surface at equidistance, 3.1 nm, from each other with a probe DNA molecule attached to the top...
The meaning of PULSE-LABEL is to cause a pulse of a radiolabeled atom or substance to become incorporated into (as a molecule or cell component). How to use pulse-label in a sentence.
Small molecules are chemical compounds with a molecular weight (MW) of less than 1 kDa. Many therapeutics and toxins fall in the category of small molecules, and therefore, the ability to measure small-molecule binding kinetics is of critical importance in pharmaceutical, diagnostic, and food indu...
During the whole procedure, L-glutamic acid acted not only as complexing and capping agent, but a bridging agent between DNA molecule and zinc selenide nanospheres. The ZnSe nanoshperes could be used for the detection of the DNA hybridization. DNA specific-sequence related to PAT gene in the ...
原理:Random decamers are annealed to a denatured template DNA molecule and new strands are synthesized by the Klenow Fragment, exo- in the presence of biotin-dUTP. During this reaction, the biotinylated nucleotides are incorporated into the newly synthesized complementary DNA strand (see Fig.1). ...
The string_express can be a Python string format pattern with atom properties as variables like 'id=%s, index=%s, name=%s" % (ID, index, name)'. For example, the following commands label 3 atoms in a methane molecule: PyMOL># load the carbon atom PyMOL>delete all PyMOL>load Carbon...
Optical molecular imaging for systems biology: from molecule to organism. Anal. Bioanal. Chem. 386, 444–457 (2006). Article PubMed PubMed Central Google Scholar Bullen, A. Microscopic imaging techniques for drug discovery. Nat. Rev. Drug Discov. 7, 54–67 (2008). Article PubMed Google ...
a processive DNA polymerase wherein the polymerase remains bound to a DNA molecule for at least 500 bases before dissociating in an environmental condition normally used in the extension reaction of a DNA sequencing reaction, the polymerase having less than 500 units of exonuclease activity per mg ...