If more than 45 mL was acquired, only 45 mL was used and the remaining sample was discarded. After clotting for 30 min, the venous whole blood was centrifuged at 1200×g for 10 min to separate serum from red blood cells. A 3–5 mL volume of serum was collected and resuspended in a...
Each blood sample was approximately 5 mL in volume and was extracted intravenously from volunteers before breakfast to avoid the interference of food. en, the blood sample was deposited at 4°C for 4 h and was centrifuged at 3000 rpm for 10 min in order to remove the blood cells, br...
Then, the supernatant of the centrifuged sample was diluted to 10 times with pH value of 7.5. The ATP was added in diluted samples at concentrations of 100 nM, 300 nM, and 500 nM, respectively. Each sample measurement was repeated in triplicate to compute the recovery. Results and ...
During the first 12 months of the study, participants were randomly assigned to one of two treatment groups. To achieve allocation concealment, randomization was independently conducted online using a web-based application. The randomization was balanced and non-stratified. In the first arm, MCI pati...
(45°). After incubation the supernatant was collected and transferred onto the Ficoll (GE healthcare) surface of a 15 ml falcon tube. Subsequently, the tube was centrifuged at room temperature at 310 × RCF for 20 min using minimum acceleration. After centrifugation, the supernatant was ...
The pellets are washed once gently with 1 ml of ice-cold wash buffer and centrifuged again. Final pellets are monitored for radioactivity and then solubilized in electrophoresis sample buffer (see below) before being subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). ...
A blood sample of 10 mL will be collected at baseline only if the centre has the necessary equipment for centrifugation and freezing the plasma. The sample will be centrifuged, and the plasma will be frozen on-site within 4 h at −80 °C. The available samples of the primary tumor and...
Lastly, TEM was used to show the uptake of 15 nm AuNPs by triple cell co-culture which consisted of human blood monocyte derived macrophages and dendritic cells as well as the A549 human alveolar epithelial cells [16]. In addition, AuNP toxicity in relation to cellular uptake has been ...
Patients worldwide require therapeutic transfusions of packed red blood cells (pRBCs), which is applied to the high-risk patients who need periodic transfusions due to leukemia, lymphoma, myeloma and other blood diseases or disorders. Contrary to the gen
incubated at −40 °C for 1 h and then centrifuged at 12,000 rpm for 15 min at 4 °C. The resulting supernatant was transferred to a fresh glass vial for analysis. The quality control (QC) sample was prepared by mixing equal aliquots of the supernatants from all the ...