Each blood sample was approximately 5 mL in volume and was extracted intravenously from volunteers before breakfast to avoid the interference of food. en, the blood sample was deposited at 4°C for 4 h and was centrifuged at 3000 rpm for 10 min in order to remove the blood cells, br...
If more than 45 mL was acquired, only 45 mL was used and the remaining sample was discarded. After clotting for 30 min, the venous whole blood was centrifuged at 1200×g for 10 min to separate serum from red blood cells. A 3–5 mL volume of serum was collected and resuspended in a...
The supernatant was collected and recentrifuged at 1000g for 10 min. The resulting supernatant was then centrifuged at 9000g for 15 min at 4 °C and resuspended in FFA-free HES buffer with 0.2% BSA. A small aliquot of purified mitochondria was reduced after the addition of a small amount...
(wash buffer), and centrifugation at 12,000gfor 5 min in a Beckman microfuge. The pellets are washed once gently with 1 ml of ice-cold wash buffer and centrifuged again. Final pellets are monitored for radioactivity and then solubilized in electrophoresis sample buffer (see below) before ...
(GDNF, GFD, SOD2, Catalase, and GGT1, among others). Participants’ blood samples were collected in an EDTA BD VACUTAINER SSTII DE. The samples were then centrifuged at 2000g for 10 min at 4 °C, and the plasma was aliquoted in SARSTEDT tubes and stored at − 80 °C. Blood ...
Among the 55 evaluable subjects with bacteremia, all achieved clearance of their blood cultures with 2 doses of dalbavancin [8,9,10, 14, 17]. Consequently, dalbavancin is an appealing alternative option for the treatment of S. aureus bacteremia without the need for prolonged central venous ...
0.12 ml of freshly prepared seed solution was added and set aside in dark for 14 hours. The solution turned from colorless to violet brown with most of the color change happening in the first hour. Prior to use, the gold nanorod solution was centrifuged at 13,000 rpm for 10 min to remo...
After adding 200 μL of UA buffer (8 M urea and 0.15 M Tris-HCl, pH 8.0), each sample was concentrated in 30 kDa Microcon filtration devices and centrifuged at 14,000g for 15 min. UA buffer (200 μL) was added, and the mixture was centrifuged at 14,000g for 15 min. Then, ...
incubated at −40 °C for 1 h and then centrifuged at 12,000 rpm for 15 min at 4 °C. The resulting supernatant was transferred to a fresh glass vial for analysis. The quality control (QC) sample was prepared by mixing equal aliquots of the supernatants from all the ...
Detection and characterization of rare circulating tumor cells (CTCs) in patients' blood is important for the diagnosis and monitoring of cancer. The traditional way of counting CTCs via fluorescent images requires a series of tedious experimental proced