For CSF scRNA-seq, CSF cells in media were centrifuged at 400 × g for 5 min and resuspended in 40 µl of X-Vivo15 media. A total of 5 µl of the single-cell suspension were manually counted in a Fuchs-Rosenthal chamber. The maximum of CSF cells used for input was...
The blood samples were centrifuged at 1200 × g for 5 min at 4 °C, and the plasma was collected. Concentrations of testosterone were assayed by employing commercial ELISAs (Solarbio, SEKSM-0003) in accordance with the manufacturer’s instructions. Sperm parameters testing Male mice ...
Reduced System (LRS) cones using Ficoll–Paque (GE Healthcare). 20ml of blood was layered on 25ml of Ficoll-Paque and centrifuged at 900×gfor 30min. The PBMC layer was mixed with RPMI in a new tube and centrifuged an additional three times, at 400×gfor 10min, 8min and 5m...
MCs were stained for PD-L1+M-MDSCs and MO/MA using fluorochrome-labeled monoclonal antibodies (mAb) against HLA-DR, CD14 and PD-L1(CD274). Representative dot plots from the blood sample of OC patient of HLA-DR−/lowCD14+ M-MDSCs, HLA-DR+CD14+ MO/MA, PD-L1-expressing M-MDSC ...
The internal standard was generated by combining equal amounts of each sample within the experiment and was labeled with Cy2. Differentially labeled samples were mixed together according to the scheme in Table S1 loaded onto Immobiline DryStrip gel strips (24 cm, pH 3 to 10 nonlinear; GE ...
All obtained fluorescence intensity data were normalized on the control sample [21]. 2.7.4. RBCs Hemolysis The level of RBCs hemolysis after 24 h co-incubation with different-size magnetic polyelectrolyte microcapsules was evaluated using murine whole-blood. For this, blood was centrifuged at 400×...
Collected supernatants were centrifuged at 10,000× g for 30 min at 4 ◦C, and the cytosolic fraction (supernatants) was collected. The mitochondrial fraction (pellets) was resuspended in the extraction buffer, and the protein amount of each fraction was measured by a bicinchoninic acid protein...
More than 1,200 lipid molecular species from a single DBS sample were identified and quantified. The lipidomics profile of the DBS samples is comparable to whole blood matrix. DBS-MS/MSALL lipidomic analysis in postprandial experiments revealed significant alterations in triacylglyceride species. Tem...
Blood samples were allowed to coagulate at room temperature or in the refrigerator for 1 h and then centrifuged at 3000 rpm for 15 min. The clear supernatant serum was transferred into dry, sterile, and labeled stopper vials and used for the clinical and biochemical tests, including liver...
To remove red blood cells (RBC), the pellet was suspended in PBS containing 0.15 M NH4Cl, 10 mM NaHCO3, and 0.1 mM EDTA, and centrifuged at 350× g for 6 min. This process was repeated twice. Pellets containing monocytes and lymphocytes were suspended in PBS and placed in 15-ml ...