A normal distribution of 3D cell structures is produced when formed through magnetic assembly. The use of this method in co-culturing of different cell lines is also demonstrated. The technique is envisioned to be transferable to other cell lines or diamagnetic biological molecules, with potential ...
A variety of super-resolution fluorescence microscopies also enable sub-diffraction imaging of cellular structures1,2, but all require labels that are specific to the problem at hand and often rely on a priori knowledge of structures for effective labelling. In practice, photobleaching of the fluoro...
In the following, by “cell mass” we will mean the dry mass of the cells. This approach to relate RI to dry mass has been used previously to study cellular growth rate14, quantifying mass densities of intracellular structures15, in addition to phenotyping and characterization of pathological ...
Currently, only super-resolution fluorescence techniques are used to study cellular structures in live cells below the diffraction limit (<200nm). However, these approaches require labeling that may alter the native cell structure and can only visualize a few molecules concurrently. Here we present ...
Histopathology and life-science research use chromatic dyes or fluorescence markers for histochemical staining to visualize tissue and cellular structures1,2. In particular, hematoxylin and eosin (H&E) staining is the gold standard for microscopic tissue examination in histopathology3. However, ...
Myelin wraps around the axon in a compact, multilayered spiral (Fig. 1). In a cross-sectional view, it forms nearly concentric rings, alternating cellular space with a high refractive index (n = 1.47) and extracellular space with a lower refractive index (n = 1.35)25,26,27,28...
Proteomics of organelles and large cellular structures Nat. Rev. Mol. Cell Biol, 6 (2005), pp. 702-714 View in ScopusGoogle Scholar 3 J.V. Olsen, B. Blagoev, F. Gnad, B. Macek, C. Kumar, P. Mortensen, M. Mann Global, in vivo, and site-specific phosphorylation dynamics in signali...
However, MSCs are scarce in the human body and are mixed with other types of cells [33], [41], [54]. Even for a purified cell population, the difference in cellular status, young and active or senescent stage, will also affect their differentiation potential and ultimately therapeutic ...
Protein aggregation is usually seen as the transition from diffuse signal to the formation of puncta or foci structures. However, as useful and valuable as FPs can be, they also have limitations. Several studies have shown that fluorescently tagged proteins exhibit altered biochemical, biophysical, ...
this method inherently requires fluorescent labeling which has several drawbacks. Here we present a label-free method for evaluating cellular drug responses only by high-throughput bright-field imaging with the aid of machine learning algorithms. Specifically, we performed high-throughput bright-field ima...